Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone
文献类型：期刊论文
| 作者 | Tojima, Takuro ; Akiyama, Hiroki ; Itofusa, Rurika ; Li, Yan ; Katayama, Hiroyuki ; Miyawaki, Atsushi ; Kamiguchi, Hiroyuki |
| 刊名 | NATURE NEUROSCIENCE
 |
| 出版日期 | 2007 |
| 卷号 | 10期号:1页码:58-66 |
| 关键词 | NERVE GROWTH FLUORESCENT PROTEIN SIGNAL-TRANSDUCTION TRPC CHANNELS RHO-GTPASES DYNAMICS FILOPODIA ADHESION NEURONS SNARE |
| ISSN号 | 1097-6256 |
| 通讯作者 | Kamiguchi, H (reprint author), RIKEN, Inst Phys & Chem Res, Brain Sci Inst, Lab Neuronal Growth Mech, 2-1 Hirosawa, Wako, Saitama 3510198, Japan,kamiguchi@brain.riken.jp |
| 英文摘要 | Asymmetric elevation of the Ca2+ concentration in the growth cone can mediate both attractive and repulsive axon guidance. Ca2+ signals that are accompanied by Ca2+-induced Ca2+ release (CICR) trigger attraction, whereas Ca2+ signals that are not accompanied by CICR trigger repulsion. The molecular machinery downstream of Ca2+ signals, however, remains largely unknown. Here we report that asymmetric membrane trafficking mediates growth cone attraction. Local photolysis of caged Ca2+, together with CICR, on one side of the growth cone of a chick dorsal root ganglion neuron facilitated the microtubule-dependent centrifugal transport of vesicles towards the leading edge and their subsequent vesicle-associated membrane-protein 2 (VAMP2)-mediated exocytosis on the side with an elevated Ca2+ concentration. In contrast, Ca2+ signals without CICR had no effect on the vesicle transport. Furthermore, pharmacological inhibition of VAMP2-mediated exocytosis prevented growth cone attraction, but not repulsion. These results strongly suggest that growth cone attraction and repulsion are driven by distinct mechanisms, rather than using the same molecular machinery with opposing polarities. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1795]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Tojima, Takuro,Akiyama, Hiroki,Itofusa, Rurika,et al. Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone[J]. NATURE NEUROSCIENCE,2007,10(1):58-66. |
| APA | Tojima, Takuro.,Akiyama, Hiroki.,Itofusa, Rurika.,Li, Yan.,Katayama, Hiroyuki.,...&Kamiguchi, Hiroyuki.(2007).Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone.NATURE NEUROSCIENCE,10(1),58-66. |
| MLA | Tojima, Takuro,et al."Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone".NATURE NEUROSCIENCE 10.1(2007):58-66. |
入库方式： OAI收割
来源：上海神经科学研究所
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