Thermal nociception in adult Drosophila: behavioral characterization and the role of the painless gene
文献类型：期刊论文
| 作者 | Xu, S. Y. ; Cang, C. L. ; Liu, X. F. ; Peng, Y. Q. ; Ye, Y. Z. ; Zhao, Z. Q. ; Guo, A. K. |
| 刊名 | GENES BRAIN AND BEHAVIOR
 |
| 出版日期 | 2006 |
| 卷号 | 5期号:8页码:602-613 |
| ISSN号 | 1601-1848 |
| 关键词 | CO2 laser Drosophila hot plate jump response mushroom body nociception painless ANTERIOR CINGULATE CORTEX CAENORHABDITIS-ELEGANS CAPSAICIN-RECEPTOR FLIGHT ORIENTATION ESCAPE CIRCUIT MUTANTS NEURONS BRAIN HABITUATION AVOIDANCE |
| 通讯作者 | Guo, AK (reprint author), Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China,akguo@ion.ac.cn |
| 英文摘要 | Nociception, warning of injury that should be avoided, serves an important protective function in animals. In this study, we show that adult Drosophila avoids noxious heat by a jump response. To quantitatively analyze this nociceptive behavior, we developed two assays. In the CO2 laser beam assay, flies exhibit this behavior when a laser beam heats their abdomens. The consistency of the jump latency in this assay meets an important criterion for a good nociceptive assay. In the hot plate assay, flies jump quickly to escape from a hot copper plate (> 45 degrees C). Our results demonstrate that, as in mammals, the latency of the jump response is inversely related to stimulus intensity, and innoxious thermosensation does not elicit this nociceptive behavior. To explore the genetic mechanisms of nociception, we examined several mutants in both assays. Abnormal nociceptive behavior of a mutant, painless(1), indicates that painless, a gene essential for nociception in Drosophila larvae, is also required for thermal nociception in adult flies. painless is expressed in certain neurons of the peripheral nervous system and thoracic ganglia, as well as in the definite brain structures, the mushroom bodies. However, chemical or genetic insults to the mushroom bodies do not influence the nociceptive behavior, suggesting that different painless-expressing neurons play diverse roles in thermal nociception. Additionally, no-bridge(KS49), a mutant that has a structural defect in the protocerebral bridge, shows defective response to noxious heat. Thus, our results validate adult Drosophila as a useful model to study the genetic mechanisms of thermal nociception. |
| 学科主题 | Behavioral Sciences ; Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1801]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xu, S. Y.,Cang, C. L.,Liu, X. F.,et al. Thermal nociception in adult Drosophila: behavioral characterization and the role of the painless gene[J]. GENES BRAIN AND BEHAVIOR,2006,5(8):602-613. |
| APA | Xu, S. Y..,Cang, C. L..,Liu, X. F..,Peng, Y. Q..,Ye, Y. Z..,...&Guo, A. K..(2006).Thermal nociception in adult Drosophila: behavioral characterization and the role of the painless gene.GENES BRAIN AND BEHAVIOR,5(8),602-613. |
| MLA | Xu, S. Y.,et al."Thermal nociception in adult Drosophila: behavioral characterization and the role of the painless gene".GENES BRAIN AND BEHAVIOR 5.8(2006):602-613. |
入库方式： OAI收割
来源：上海神经科学研究所
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