中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Chloride homeostasis differentially affects GABA(A) receptor- and glycine receptor-mediated effects on spontaneous circuit activity in hippocampal cell culture

文献类型:期刊论文

作者Wang, Wei
刊名NEUROSCIENCE LETTERS
出版日期2006
卷号406期号:40910页码:41229
关键词KCC2 GABA(A) receptor glycine receptor tonic current spontaneous circuit activity hippocampal neuron CEREBELLAR GRANULE CELLS SHUNTING INHIBITION COTRANSPORTER KCC2 GABA(A) RECEPTORS CROSS-INHIBITION RAT HIPPOCAMPUS NEURONS ACTIVATION TRANSMISSION OSCILLATIONS
ISSN号0304-3940
通讯作者Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
英文摘要The potassium-chloride cotransporter 2 (KCC2)-dependent intracellular chloride level determines whether neurons respond to GABA and/or glycine by depolarization or hyperpolarization. However, still unknown is the role of KCC2-dependent chloride homeostasis in regulating the spontaneous activity of neuronal circuits via GABA(A) receptor (GABA(A)R) and the glycine receptor (GlyR). In this study, patch-clamp recordings were performed to measure the change of spontaneous neuronal activity in cultured hippocampal neurons. Our results showed that inhibition of KCC2 with furosemide, as well as blockade of GABA(A)R with bicuculline, significantly enhanced circuit activity. Perfusion with bicuculline further enhanced the effects of furosemide on spontaneous circuit activity, while furosemide did not alter the effects of bicuculline. Surprisingly, blockade of GlyR not only induced obvious tonic currents, but also significantly decreased spontaneous synaptic activity. Moreover, inhibition of KCC2 did not change the depressive effect of strychnine on neuronal circuits. Our findings suggest that KCC2-dependent chloride homeostasis is mainly involved in GABA(A)R-mediated synaptic inhibition whereas GlyR-mediated tonic action plays a totally different role in regulating hippocampal circuit activity. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
学科主题Neurosciences & Neurology
收录类别SCI
语种英语
公开日期2012-07-23
源URL[http://ir.sibs.ac.cn/handle/331001/1803]  
专题上海神经科学研究所_神经所(总)
上海神经科学研究所_视知觉机制研究组
推荐引用方式
GB/T 7714
Wang, Wei. Chloride homeostasis differentially affects GABA(A) receptor- and glycine receptor-mediated effects on spontaneous circuit activity in hippocampal cell culture[J]. NEUROSCIENCE LETTERS,2006,406(40910):41229.
APA Wang, Wei.(2006).Chloride homeostasis differentially affects GABA(A) receptor- and glycine receptor-mediated effects on spontaneous circuit activity in hippocampal cell culture.NEUROSCIENCE LETTERS,406(40910),41229.
MLA Wang, Wei."Chloride homeostasis differentially affects GABA(A) receptor- and glycine receptor-mediated effects on spontaneous circuit activity in hippocampal cell culture".NEUROSCIENCE LETTERS 406.40910(2006):41229.

入库方式: OAI收割

来源:上海神经科学研究所

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