Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses
文献类型：期刊论文
| 作者 | Zhong, Weixia ; Dong, Zhifang ; Tian, Meng ; Cao, Jun ; Xu, Tianle(徐天乐） ; Xu, Lin ; Luo, Jianhong |
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2006 |
| 卷号 | 79期号:9页码:861-869 |
| 关键词 | AMPA receptor phosphorylation CaMKII alpha opiate withdrawal synaptosome LONG-TERM POTENTIATION PROTEIN-KINASE-II VENTRAL TEGMENTAL AREA RAT CEREBRAL-CORTEX SYNAPTIC PLASTICITY CHRONIC MORPHINE GLUCOCORTICOID-RECEPTOR STRESS COCAINE TRAFFICKING |
| ISSN号 | 0024-3205 |
| 通讯作者 | Luo, JH (reprint author), Zhejiang Univ, Sch Med, Dept Neurobiol, Hangzhou 310006, Peoples R China,lxu@vip.163.com |
| 英文摘要 | Adaptive changes in brain areas following drug withdrawal are believed to contribute to drug seeking and relapse. Cocaine withdrawal alters the expression of GluR1 and GluR2/3 subunits of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors in nucleus accumbens or amygdala, but the influence of drug withdrawal on hippocampus is little known. Here, we have examined the expression of GluR1 and GluR2/3 in hippocampal membrane and synaptic fractions following repeated morphine exposure and subsequent withdrawal. Repeated morphine exposure for 12 d increased GluR1 and GluR2/3 in synaptosome but not in membrane fraction. Interestingly, CaMKII alpha, known to be able to regulate the function of AMPA receptors, was decreased in synaptosome but not in membrane fraction; pCaMKII alpha, the phosphorylated form of CaMKII alpha, was increased in both fractions. However, during opiate withdrawal, GluR1 was generally reduced while GluR2/3 was prominently increased in both fractions; pCaMKII alpha was strongly decreased immediately after withdrawal, but detectably increased in late phase of morphine withdrawal in both fractions. Importantly, the opiate withdrawal-induced increase in GluR2/3 was dependent on the activation of glucocorticoid receptors and NMDA receptors, as it was prevented by the glucocorticoid receptor antagonist RU38486, or intrahippocampal injection of the NMDA receptor antagonist AP-5 or the antagonist to NR2B-containing NMDA receptors, Ro25-6981. These findings indicate that opiate withdrawal induces dynamic expression of GluR1 and GluR2/3 subunits of AMPA receptors in hippocampal synapses, possibly revealing an adaptive process of the hippocampal functions following opiate withdrawal. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1811]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zhong, Weixia,Dong, Zhifang,Tian, Meng,et al. Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses[J]. LIFE SCIENCES,2006,79(9):861-869. |
| APA | Zhong, Weixia.,Dong, Zhifang.,Tian, Meng.,Cao, Jun.,Xu, Tianle.,...&Luo, Jianhong.(2006).Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses.LIFE SCIENCES,79(9),861-869. |
| MLA | Zhong, Weixia,et al."Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses".LIFE SCIENCES 79.9(2006):861-869. |
入库方式： OAI收割
来源：上海神经科学研究所
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