Kiss-and-run glutamate secretion in cultured and freshly isolated rat hippocampal astrocytes
文献类型：期刊论文
| 作者 | Chen, XK ; Wang, LC ; Zhou, Y ; Zheng, LH ; Zhou, Z(周专) |
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2005 |
| 卷号 | 25期号:40页码:9236-9243 |
| 关键词 | kiss-and-run fusion pore amperometry single-vesicle FM imaging glutamate freshly isolated astrocyte ADRENAL CHROMAFFIN CELLS SINGLE SYNAPTIC VESICLES AMPEROMETRIC DETECTION TRANSMITTER RELEASE QUANTAL SECRETION FUSION GLIA EXOCYTOSIS NEURONS CALCIUM |
| ISSN号 | 0270-6474 |
| 通讯作者 | Zhou, Z (reprint author), Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China,zzhou@pku.edu.cn |
| 英文摘要 | Under physiological conditions, astrocytes not only passively support and nourish adjacent neurons, but also actively modulate neuronal transmission by releasing "glial transmitters," such as glutamate, ATP, and D-serine. Unlike the case for neurons, the mechanisms by which glia release transmitters are essentially unknown. Here, by using electrochemical amperometry and frequency-modulated single-vesicle imaging, we discovered that hippocampal astrocytes exhibit two modes of exocytosis of glutamate in response to various stimuli. After physiological stimulation, a glial vesicle releases a quantal content that is only 10% of that induced by nonphysiological, mechanical stimulation. The small release event arises from a brief (similar to 2 ms) opening of the fusion pore. We conclude that, after physiological stimulation, astrocytes release glutamate via a vesicular "kiss-and-run" mechanism. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1880]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Chen, XK,Wang, LC,Zhou, Y,et al. Kiss-and-run glutamate secretion in cultured and freshly isolated rat hippocampal astrocytes[J]. JOURNAL OF NEUROSCIENCE,2005,25(40):9236-9243. |
| APA | Chen, XK,Wang, LC,Zhou, Y,Zheng, LH,&Zhou, Z.(2005).Kiss-and-run glutamate secretion in cultured and freshly isolated rat hippocampal astrocytes.JOURNAL OF NEUROSCIENCE,25(40),9236-9243. |
| MLA | Chen, XK,et al."Kiss-and-run glutamate secretion in cultured and freshly isolated rat hippocampal astrocytes".JOURNAL OF NEUROSCIENCE 25.40(2005):9236-9243. |
入库方式： OAI收割
来源：上海神经科学研究所
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