SNAP-29-mediated modulation of synaptic transmission in cultured hippocampal neurons
文献类型：期刊论文
| 作者 | Pan, PY ; Cai, Q ; Lin, L ; Lu, PH ; Duan, SM ; Sheng, ZH |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 280期号:27页码:25769-25779 |
| 关键词 | PROTEIN-PROTEIN INTERACTIONS SHORT-TERM PLASTICITY NEUROTRANSMITTER RELEASE CALCIUM-CHANNELS N-TYPE PRESYNAPTIC PROTEINS CENTRAL SYNAPSES NERVE-TERMINALS ALPHA-SNAP IN-VITRO |
| ISSN号 | 0021-9258 |
| 通讯作者 | Duan, SM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,shumin@ion.ac.cn ; shengz@ninds.nih.gov |
| 英文摘要 | Identifying the molecules that regulate both the recycling of synaptic vesicles and the SNARE components required for fusion is critical for elucidating the molecular mechanisms underlying synaptic plasticity. SNAP- 29 was initially isolated as a syntaxin- binding and ubiquitously expressed protein. Previous studies have suggested that SNAP- 29 inhibits SNARE complex disassembly, thereby reducing synaptic transmission in cultured superior cervical ganglion neurons in an activity-dependent manner. However, the role of SNAP- 29 in regulating synaptic vesicle recycling and short- term plasticity in the central nervous system remains unclear. In the present study, we examined the effect of SNAP- 29 on synaptic transmission in cultured hippocampal neurons by dual patch clamp whole- cell recording, FM dye imaging, and immunocytochemistry. Our results demonstrated that exogenous expression of SNAP- 29 in presynaptic neurons significantly decreased the efficiency of synaptic transmission after repetitive firing within a few minutes under low and moderate frequency stimulations ( 0.1 and 1 Hz). In contrast, SNAP- 29 did not affect the density of synapses and basal synaptic transmission. Whereas neurotransmitter release was unaffected during intensive stimulation, recovery after synaptic depression was impaired by SNAP- 29. Furthermore, knockdown of SNAP- 29 expression in neurons by small interfering RNA increased the efficiency of synaptic transmission during repetitive firing. These findings suggest that SNAP- 29 acts as a negative modulator for neurotransmitter release, probably by slowing recycling of the SNARE- based fusion machinery and synaptic vesicle turnover. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1894]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Pan, PY,Cai, Q,Lin, L,et al. SNAP-29-mediated modulation of synaptic transmission in cultured hippocampal neurons[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(27):25769-25779. |
| APA | Pan, PY,Cai, Q,Lin, L,Lu, PH,Duan, SM,&Sheng, ZH.(2005).SNAP-29-mediated modulation of synaptic transmission in cultured hippocampal neurons.JOURNAL OF BIOLOGICAL CHEMISTRY,280(27),25769-25779. |
| MLA | Pan, PY,et al."SNAP-29-mediated modulation of synaptic transmission in cultured hippocampal neurons".JOURNAL OF BIOLOGICAL CHEMISTRY 280.27(2005):25769-25779. |
入库方式： OAI收割
来源：上海神经科学研究所
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