Suppression of Pyk2 attenuated the increased tyrosine phosphorylation of NMDA receptor subunit 2A after brain ischemia in rat hippocampus
文献类型：期刊论文
| 作者 | Liu, Y ; Zhang, GY ; Yan, JZ ; Xu, TL(徐天乐) |
| 刊名 | NEUROSCIENCE LETTERS
 |
| 出版日期 | 2005 |
| 卷号 | 379期号:1页码:55-58 |
| 关键词 | brain ischemia NMDA receptor Pyk2 tyrosine phosphorylation Src D-ASPARTATE RECEPTOR MOLECULAR-MECHANISMS SRC KINASE ACTIVATION DOMAINS COMPLEX PSD-95 INJURY FYN |
| ISSN号 | 0304-3940 |
| 通讯作者 | Zhang, GY (reprint author), Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol & Biophys, Hefei 230027, Peoples R China,gyzhang@xzmc.edu.cn |
| 英文摘要 | Effects of suppressing the protein expression of Pyk2 on increased tyrosine phosphorylation of N-methyl-D-aspartate (NMDA) receptor subunit 2A (NR2A) after brain ischemia in rat hippocampus were studied with immunoprecipitation and immunoblot. Transient (15 min) brain ischemia and reperfusion (I/R) was induced by four-vessel occlusion in Sprague-Dawley (SD) rats. I/R led to increases of tyrosine phosphorylation of NR2A and interaction of Pyk2 and Src kinase with NR2A after 6 h of reperfusion. The increases were attenuated by Pyk2 antisense oligonucleotides intracerebroventricularly infused every 24 h for 4 days before ischemia, but not missense oligonucleotides or vehicle. The antisense also inhibited the increased auto-phosphorylation of Pyk2 and Src kinase, while the protein expression of NR2A or Src kinase had no obvious change under the above conditions. The data suggested that Pyk2 may be involved in facilitating NR2A tyrosine phosphorylation by Src kinase after I/R. (c) 2004 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1907]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Liu, Y,Zhang, GY,Yan, JZ,et al. Suppression of Pyk2 attenuated the increased tyrosine phosphorylation of NMDA receptor subunit 2A after brain ischemia in rat hippocampus[J]. NEUROSCIENCE LETTERS,2005,379(1):55-58. |
| APA | Liu, Y,Zhang, GY,Yan, JZ,&Xu, TL.(2005).Suppression of Pyk2 attenuated the increased tyrosine phosphorylation of NMDA receptor subunit 2A after brain ischemia in rat hippocampus.NEUROSCIENCE LETTERS,379(1),55-58. |
| MLA | Liu, Y,et al."Suppression of Pyk2 attenuated the increased tyrosine phosphorylation of NMDA receptor subunit 2A after brain ischemia in rat hippocampus".NEUROSCIENCE LETTERS 379.1(2005):55-58. |
入库方式： OAI收割
来源：上海神经科学研究所
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