Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila
文献类型：期刊论文
| 作者 | Xu, TX ; Gong, N ; Xu, TL(徐天乐) |
| 刊名 | JOURNAL OF NEUROGENETICS
 |
| 出版日期 | 2005 |
| 卷号 | 19期号:2页码:87-107 |
| 关键词 | I-A A-type K+ channels Cd2+ Zn2+ Ca2+ and Mg2+ ions slo(1) Sh(M) mutations larval neurons whole-cell patch-clamp TRANSIENT OUTWARD CURRENT NEGATIVE SURFACE-CHARGE VENTRICULAR MYOCYTES K+ CHANNELS ZINC MODULATION BINDING-SITE RAT CALCIUM CURRENTS SHAKER |
| ISSN号 | 0167-7063 |
| 通讯作者 | Xu, TL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Key Lab Neurobiol, 320 Yueyang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn |
| 英文摘要 | Drosophila mutants provide an ideal model to study channel-type specificity of ion channel regulation in situ. In this study, the effects of divalent cations on voltage-gated K+ currents were investigated in acutely dissociated central neurons of Drosophila third instar larvae using the whole-cell patch-clamp recording. Our data showed that micromolar Cd2+ enhanced the peak inactivating current (I-A) without affecting the delayed component (I-K ). The same results were obtained in Ca2+-free external solution, and from slo(1) mutation, which eliminates transient Ca2+-activated K+ current. Micromolar d +and Zn, and millimolar Ca and Mg all shifted the steady-state inactivation curve Of IA without affecting the voltage-dependence of I-A activation, whereas millimolar Cd2+ markedly affected both the activation and steady-state inactivation curves for IA. Divalent cations affected IA with different potency; the sequence was: Zn2+ > Cd2+ > Ca2+ > Mg2+. The modulation of I-A by Cd2+ was partially inhibited in Sh(M), a null Shaker (one of I-A-encoding genes) mutation. Taken together, the channel-type specificity, the asymmetric effects on IA activation and inactivation kinetics, and the diverse potency of divalent cations all strongly support the idea that physiological divalent cations modulate A-type K+ channels through specific binding to extracellular sites of the channels. |
| 学科主题 | Genetics & Heredity ; Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1913]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xu, TX,Gong, N,Xu, TL. Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila[J]. JOURNAL OF NEUROGENETICS,2005,19(2):87-107. |
| APA | Xu, TX,Gong, N,&Xu, TL.(2005).Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila.JOURNAL OF NEUROGENETICS,19(2),87-107. |
| MLA | Xu, TX,et al."Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila".JOURNAL OF NEUROGENETICS 19.2(2005):87-107. |
入库方式： OAI收割
来源：上海神经科学研究所
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