Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments
文献类型：期刊论文
| 作者 | Wu, B ; Wang, YM ; Xiong, W ; Zheng, LH ; Fu, CL ; Bruce, IC ; Zhang, C ; Zhou, Z |
| 刊名 | FRONTIERS IN BIOSCIENCE
 |
| 出版日期 | 2005 |
| 卷号 | 10页码:761-767 |
| 关键词 | puffer patch clamp solution exchange perfusion drug delivery ROOT GANGLION NEURONS SECRETION CHANNELS CELLS |
| ISSN号 | 1093-9946 |
| 通讯作者 | Zhou, Z (reprint author), Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China,zzhou@pku.edu.cn |
| 英文摘要 | In biological experiments, especially in neuroscience research, it is important to manipulate the extracellular environment efficiently. We have developed a micro-puffing system for local drug delivery to single cells in electrophysiological experiments, and validated the kinetic properties of this instrument. Based on our results, the kinetics of the delivery of solutions and the territory controlled by this system are influenced by several factors: ( 1) inner diameter ( I. D.) of the guide tubing; ( 2) I. D. of the puffing tip; ( 3) angle of the puffing tip; and ( 4) gravity or external pressure applied to the solution. The system can fully control a territory of 200 x 600 mu m(2). The minimum delay in response to drug delivery is 10-20 ms. Switching between different solutions takes less than 100 ms. The minimum volume of solution required by the system is 0.2 ml. Taken together, our results provide useful data for designing and using an efficient drug/solution delivery system in electrophysiological experiments. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1926]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Wu, B,Wang, YM,Xiong, W,et al. Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments[J]. FRONTIERS IN BIOSCIENCE,2005,10:761-767. |
| APA | Wu, B.,Wang, YM.,Xiong, W.,Zheng, LH.,Fu, CL.,...&Zhou, Z.(2005).Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments.FRONTIERS IN BIOSCIENCE,10,761-767. |
| MLA | Wu, B,et al."Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments".FRONTIERS IN BIOSCIENCE 10(2005):761-767. |
入库方式： OAI收割
来源：上海神经科学研究所
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