Preparation of single rice chromosome for construction of a DNA library using a laser microbeam trap
文献类型：期刊论文
| 作者 | Liu, XH ; Wang, HW ; Li, YM ; Tang, YS ; Liu, YL ; Hu, X ; Jia, PX ; Ying, K ; Feng, Q ; Guan, JP ; Jin, CQ ; Zhang, L ; Lou, LR ; Zhou, ZA ; Han, B |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 109期号:3页码:217-226 |
| 关键词 | optical scissors optical trap single rice chromosome adaptor PCR GENETICS CELLS TOOL |
| ISSN号 | 0168-1656 |
| 通讯作者 | Han, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Gene Res, Shanghai 200233, Peoples R China,bhan@ncgr.ac.cn |
| 英文摘要 | We report the development of a laser micromanipulation system and its application in the isolation of individual rice chromosomes directly from a metaphase cell. Microdissection and flow sorting are two major methods for the isolation of single chromosome. These methods are dependent on the techniques of chromosome spread and chromosome suspension, respectively. In the development of this system, we avoided using chromosome spread and cell suspension was used instead. The cell wall of metaphase rice cell was cut by optical scissors. The released single chromosome was captured by an optical trap and transported to an area without cell debris. The isolated single chromosome was then collected and specific library was constructed by linker adaptor PCR. The average insert size of the library was about 300bp. Two hundred inserts of chromosome 4 library were sequenced, and 96.5% were aligned to the corresponding sequences of rice chromosome 4. These results suggest the possible application of this method for the preparation of other subcellular structures and for the cloning of single macromolecule through a laser microbeam trap. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1965]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Liu, XH,Wang, HW,Li, YM,et al. Preparation of single rice chromosome for construction of a DNA library using a laser microbeam trap[J]. JOURNAL OF BIOTECHNOLOGY,2004,109(3):217-226. |
| APA | Liu, XH.,Wang, HW.,Li, YM.,Tang, YS.,Liu, YL.,...&Han, B.(2004).Preparation of single rice chromosome for construction of a DNA library using a laser microbeam trap.JOURNAL OF BIOTECHNOLOGY,109(3),217-226. |
| MLA | Liu, XH,et al."Preparation of single rice chromosome for construction of a DNA library using a laser microbeam trap".JOURNAL OF BIOTECHNOLOGY 109.3(2004):217-226. |
入库方式： OAI收割
来源：上海神经科学研究所
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