Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion
文献类型：期刊论文
| 作者 | Yu, X ; Duan, KL ; Shang, CF ; Yu, HG ; Zhou, Z |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2004 |
| 卷号 | 101期号:4页码:1051-1056 |
| 关键词 | ADRENAL CHROMAFFIN CELLS NUCLEOTIDE-GATED CHANNELS LONG-TERM POTENTIATION RECEPTOR CHANNELS PACEMAKER CHANNELS SYNAPTIC-TRANSMISSION VENTRICULAR MYOCYTES FACILITATION PERMEATION EXPRESSION |
| ISSN号 | 0027-8424 |
| 通讯作者 | Zhou, Z (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zzhou@ion.ac.cn |
| 英文摘要 | The hyperpolarization-activated cation channels (I-h) play a distinct role in rhythmic activities in a variety of tissues, including neurons and cardiac cells. in the present study, we investigated whether Ca2+ can permeate through the hyperpolarization-activated pacemaker channels (HCN) expressed in HEK293 cells and I-h channels in dorsal root ganglion (DRG) neurons. Using combined measurements of whole-cell currents and fura-2 Ca2+ imaging, we found that there is a Ca2(+) influx in proportion to I-h induced by hyperpolarization in HEK293 cells. The I-h channel blockers Cs+ and ZD7288 inhibit both HCN current and Ca2(+) influx. Measurements of the fractional Ca2+ current showed that it constitutes 0.60+/-0.02% of the net inward current through HCN4 at -120 mV. This fractional current is similar to that of the low Ca2+-permeable AMPA-R (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) channels in Purkinje neurons. In DRG neurons, activation of I-h for 30 s also resulted in a Ca2+ influx and an elevated action potential-induced secretion, as assayed by the increase in membrane capacitance. These results suggest a functional significance for I-h channels in modulating neuronal secretion by permitting Ca2+ influx at negative membrane potentials. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1978]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Yu, X,Duan, KL,Shang, CF,et al. Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2004,101(4):1051-1056. |
| APA | Yu, X,Duan, KL,Shang, CF,Yu, HG,&Zhou, Z.(2004).Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,101(4),1051-1056. |
| MLA | Yu, X,et al."Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101.4(2004):1051-1056. |
入库方式： OAI收割
来源：上海神经科学研究所
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