Martentoxin, a novel K+ channel-blocking peptide: purification, cDNA and genomic cloning, and electrophysiological and pharmacological characterization
文献类型：期刊论文
| 作者 | Ji, YH ; Wang, WX ; Ye, JG ; He, LL ; Li, YJ ; Yan, YP ; Zhou, Z |
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 84期号:2页码:325-335 |
| 关键词 | biosensor binding assay Ca2+-activated K+ channels genomic organization martentoxin patch-clamp recording ACTIVATED POTASSIUM CHANNEL ANDROCTONUS-MAURETANICUS-MAURETANICUS SCORPION PANDINUS IMPERATOR ADRENAL CHROMAFFIN CELLS BUTHUS-MARTENSI BINDING-SITES BETA-SUBUNIT RAT-BRAIN VENOM CHARYBDOTOXIN |
| ISSN号 | 0022-3042 |
| 通讯作者 | Ji, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Physiol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China, |
| 英文摘要 | Martentoxin, a novel K+-channel-specific peptide has-been purified and characterized from the venom of the East-Asian scorpion (Buthus martensi Karsch). The whole cDNA precursor sequence suggested that martentoxin was composed of 37 residues with a unique sequence compared with other scorpion neurotoxins. The genomic DNA of martentoxin showed an additional intron situated unexpectedly in the 5' UTR region, besides one located close to the C-terminal of the signal peptide. The patch-clamp recording found that martentoxin at the applied dose of 100 nM could strongly block large-conductance Ca2+-activated K+ (BKCa) currents in adrenal medulla chromaffin cells, and BKCa currents blocked by martentoxin could be fully recovered within 30 seconds after washing, which is at least 10 times faster than recovery after charybdotoxin. Meanwhile, a biosensor binding assay showed a fast association rate and a slow dissociation rate of martentoxin binding on rat brain synaptosomes. The binding of martentoxin on rat brain synaptosomes could be inhibited regularly by charybdotoxin, and gradually by toosendanin in a concentration-dependent manner, but hot by either apamin or P03 from Buthus martensi. The results thus indicate that martentoxin is a new member in the family of K+-channel-blocking ligands. |
| 学科主题 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2031]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Ji, YH,Wang, WX,Ye, JG,et al. Martentoxin, a novel K+ channel-blocking peptide: purification, cDNA and genomic cloning, and electrophysiological and pharmacological characterization[J]. JOURNAL OF NEUROCHEMISTRY,2003,84(2):325-335. |
| APA | Ji, YH.,Wang, WX.,Ye, JG.,He, LL.,Li, YJ.,...&Zhou, Z.(2003).Martentoxin, a novel K+ channel-blocking peptide: purification, cDNA and genomic cloning, and electrophysiological and pharmacological characterization.JOURNAL OF NEUROCHEMISTRY,84(2),325-335. |
| MLA | Ji, YH,et al."Martentoxin, a novel K+ channel-blocking peptide: purification, cDNA and genomic cloning, and electrophysiological and pharmacological characterization".JOURNAL OF NEUROCHEMISTRY 84.2(2003):325-335. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。