Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin
文献类型：期刊论文
| 作者 | Wang, CY ; Yang, F ; He, XP ; Je, HS ; Zhou, JZ ; Eckermann, K ; Kawamura, D ; Feng, L ; Shen, L ; Lu, B |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2002 |
| 卷号 | 277期号:12页码:10614-10625 |
| 关键词 | NERVE-MUSCLE INTERACTIONS LONG-TERM POTENTIATION GDNF-FAMILY MOTOR-NEURONS DEVELOPING MOTONEURONS HIPPOCAMPAL-NEURONS TRANSMITTER RELEASE QUANTAL SECRETION CORTICAL-NEURONS VISUAL-CORTEX |
| ISSN号 | 0021-9258 |
| 通讯作者 | Lu, B (reprint author), NICHHD, NIH, Unit Synapse Dev & Plast, Bldg 49,Rm 6A-80,49 Convent Dr,MSC4480, Washington, DC 20052 USA, |
| 英文摘要 | Glial cell line-derived neurotrophic factor (GDNF) is known for its potent effect on neuronal survival, but its role in the development and function of synapses is not well studied. Using Xenopus nerve-muscle co-cultures, we show that GDNF and its family member neurturin (NRTN) facilitate the development of the neuromuscular junction (NMJ). Long-term application of GDNF significantly increased the total length of neurites in the motoncurons. GDNF also caused an increase in the number and the size of synaptic vesicle clustering, as demonstrated by synaptobrevin-GFP fluorescent imaging, and FM dye staining. Electrophysiological experiments revealed two effects of GDNF on synaptic transmission at NMJ. First, GDN-F markedly increased the frequency of spontaneous transmission and decreased the variability of evoked transmission, suggesting an enhancement of transmitter secretion. Second, GDNF elicited a small increase in the quantal size, without affecting the average rise and decay times of synaptic currents. Imaging analysis showed that the size of acetylcholine receptor clusters at synapses increased in muscle cells overexpressing GDNF. Neurturin had very similar effects as GDNF. These results suggest that GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse through both pre- and postsynaptic mechanisms. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2064]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Wang, CY,Yang, F,He, XP,et al. Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2002,277(12):10614-10625. |
| APA | Wang, CY.,Yang, F.,He, XP.,Je, HS.,Zhou, JZ.,...&Lu, B.(2002).Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin.JOURNAL OF BIOLOGICAL CHEMISTRY,277(12),10614-10625. |
| MLA | Wang, CY,et al."Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin".JOURNAL OF BIOLOGICAL CHEMISTRY 277.12(2002):10614-10625. |
入库方式： OAI收割
来源：上海神经科学研究所
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