Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors
文献类型：会议论文
| 作者 | Liu, Yafeng ; Zhao, Yuan ; Lv, Xiaohua ; Li, Yiding ; Zhang, Xiaohui ; Zhang, Jie ; Wang, Liping ; Zen, Shaoqun |
| 出版日期 | 2012 |
| 会议名称 | REVIEW OF SCIENTIFIC INSTRUMENTS |
| 会议日期 | 2012 |
| 会议地点 | china |
| 关键词 | 2-PHOTON EXCITATION CHANNELRHODOPSIN-2 OPTOGENETICS LIGHT |
| 卷号 | 83 |
| 期号 | 2 |
| 页码 | 25116-25116 |
| 通讯作者 | Zen, SQ(reprint author), Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Peoples R China,sqzeng@mail.hust.edu.cn |
| 英文摘要 | Recently developed optogenetics provides a fast, non-invasive, and efficient method for cell activation. However, it is difficult for the optical stimulators used for optogenetics to realize selective multisite fast activation. In this paper, we developed a random-access photostimulator based on a pair of perpendicularly oriented acousto-optic deflectors. Precise laser targeting in the x-y plane was verified, and the lateral spatial resolution of laser intensity after the objective was measured as similar to 1.38 mu m. Photostimulaton of ChETA-expressing astrocytes induced reliable inward currents only if the laser beam was directed onto the targeted cell. In the ChR2-expresing neuron, multiple locations along two dendrites were stimulated, and spatiotemporal integration was observed in the soma with fast multi-site activation. These results demonstrated that this random-access photostimulator would be a powerful tool for selective multi-site fast activation. The compact and modular design of this photostimulator makes it easily integrated with different commercial microscopes, and thus widely popularized in many laboratories. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3689954] |
| 收录类别 | SCI |
| 会议主办者 | NSFC[30927001, 30925013] |
| 会议录 | REVIEW OF SCIENTIFIC INSTRUMENTS
 |
| 学科主题 | Instruments & Instrumentation ; Physics |
| 语种 | 英语 |
| ISSN号 | 0034-6748 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1501]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Liu, Yafeng,Zhao, Yuan,Lv, Xiaohua,et al. Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors[C]. 见:REVIEW OF SCIENTIFIC INSTRUMENTS. china. 2012. |
入库方式： OAI收割
来源：上海神经科学研究所
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