The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.
文献类型：期刊论文
| 作者 | Zhou, X ; Tan, DY ; Lai, JH ; Zan, RG |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 1998 |
| 卷号 | 25期号:5页码:472-475 |
| 关键词 | hepatitis A virus H-2 strain full length RNA RT-PCR amplification |
| ISSN号 | 1000-3282 |
| 英文摘要 | A method for RT-PCR amplifying long fragments cDNA was established and the 7.4 kb full-length cDNA of Hepatitis A virus (HAV) H-2 strain was amplifyed by reverse transcription and polymerase chain reaction (RT-PCR). HAV H-2 was precipitated by anti-H-2 serum specifically, then the RNA of HAV H-2 was isolated from this precipitation by acid guanidinium hydrochloride-phenal-chloroform extraction. The first-strand of HAV H-2 cDNA was synthesised by reverse transcriptase without RNase H activity, then was amplified by PCR using the 32mer primer and the Tag DNA polymerase with Deep Vent DNA polymerase. For obtaining longer PCR products, it is necessary to prepare high quality RNA and employ the longer primers and special Tag DNA polymerase. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| 公开日期 | 2012-10-18 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/15576]  |
| 专题 | 昆明植物研究所_离退休 |
| 推荐引用方式 GB/T 7714 | Zhou, X,Tan, DY,Lai, JH,et al. The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,1998,25(5):472-475. |
| APA | Zhou, X,Tan, DY,Lai, JH,&Zan, RG.(1998).The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,25(5),472-475. |
| MLA | Zhou, X,et al."The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 25.5(1998):472-475. |
入库方式： OAI收割
来源：昆明植物研究所
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