In vitro assay for human thyroid hormone receptor beta agonist and antagonist effects of individual polychlorinated naphthalenes and Halowax mixtures
文献类型：期刊论文
| 作者 | Li Na ; Ma Mei ; Wang ZiJian ; Kumaran, Senthil Satyanarayanan |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 56期号:6页码:508—513 |
| 中文摘要 | Polychlorinated naphthalenes (PCNs) are dioxin-like environmental contaminants. There is growing concern over the endocrine-disrupting effects of PCNs, but very few studies have investigated the effect of PCNs on the thyroid system. This study used a yeast two-hybrid assay, which included the recombinant human thyroid receptor(TR)-beta and reporter genes, to characterize the TR beta-disrupting effects of five individual PCN congeners, five PCN Halowax mixtures, and naphthalene. Their agonist and antagonist effects were studied in the absence and presence of 5x10(-7) mol/L 3,3',5-triiodo-L-thyronine, which induced submaximal beta-galactosidase activity. Naphthalene, 1,2,3,4,5,6,7,8-octachloronaphthalene and all of the Halowax mixtures (Halowax 1000, 1001, 1013, 1014 and 1099) showed no agonist or antagonist activity on TR beta at the concentrations tested (up to 10(-2) g/L). The lighter PCN congeners, namely 1-chloronaphthalene, 2-chloronaphthalene, 1,4-dichloronaphthalene and 1,2,3,4-tetrachloronaphthalene showed no agonist activity but showed significant antagonist activity on TR beta. The 20% relative inhibitory concentrations of these PCNs were less than 9.13 x 10(-3) g/L. Thus, bioaccumulation of these lighter PCN congeners may disrupt the thyroid hormone system and inhibit TR-mediated cellular responses. Studies in the future should investigate the possible associations between the presence PCNs and adverse health outcomes. |
| WOS记录号 | WOS:000287705400008 |
| 公开日期 | 2012-10-18 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7103]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li Na,Ma Mei,Wang ZiJian,et al. In vitro assay for human thyroid hormone receptor beta agonist and antagonist effects of individual polychlorinated naphthalenes and Halowax mixtures[J]. CHINESE SCIENCE BULLETIN,2011,56(6):508—513. |
| APA | Li Na,Ma Mei,Wang ZiJian,&Kumaran, Senthil Satyanarayanan.(2011).In vitro assay for human thyroid hormone receptor beta agonist and antagonist effects of individual polychlorinated naphthalenes and Halowax mixtures.CHINESE SCIENCE BULLETIN,56(6),508—513. |
| MLA | Li Na,et al."In vitro assay for human thyroid hormone receptor beta agonist and antagonist effects of individual polychlorinated naphthalenes and Halowax mixtures".CHINESE SCIENCE BULLETIN 56.6(2011):508—513. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。