Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway
文献类型：期刊论文
| 作者 | Zhao, Yaru; Yang, Jianming; Qin, Bo; Li, Yonghao; Sun, Yuanzhang; Su, Sizheng; Xian, Mo
|
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 90期号:6页码:1915-1922 |
| 关键词 | Isoprene Biosynthesis Escherichia coli MEP pathway |
| 中文摘要 | Isoprene is an aviation fuel of high quality and an important polymer building block in the synthetic chemistry industry. In light of high oil prices, sustained availability, and environmental concerns, isoprene from renewable materials is contemplated as a substitute for petroleum-based product. Escherichia coli with advantages over other wild microorganisms, is considered as a powerful host for biofuels and chemicals. Here, we constructed a synthetic pathway of isoprene in E. coli by introducing an isoprene synthase (ispS) gene from Populus nigra, which catalyzes the conversion of dimethylallyl diphosphate (DMAPP) to isoprene. To improve the isoprene production, we overexpressed the native 1-deoxy-D: -xylulose-5-phosphate (DXP) synthase gene (dxs) and DXP reductoisomerase gene (dxr) in E. coli, which catalyzed the first step and the second step of MEP pathway, respectively. The fed-batch fermentation results showed that overexpression of DXS is helpful for the improvement of isoprene production. Surprisingly, heterologous expression of dxs and dxr from Bacillus subtilis in the E. coli expressing ispS resulted in a 2.3-fold enhancement of isoprene production (from 94 to 314 mg/L). The promising results showed that dxs and dxr from B. subtilis functioned more efficiently on the enhancement of isoprene production than native ones. This could be caused by the consequence of great difference in protein structures of the two original DXSs. It could be practical to produce isoprene in E. coli via MEP pathway through metabolic engineering. This work provides an alternative way for production of isoprene by engineered E. coli via MEP pathway through metabolic engineering. |
| 英文摘要 | Isoprene is an aviation fuel of high quality and an important polymer building block in the synthetic chemistry industry. In light of high oil prices, sustained availability, and environmental concerns, isoprene from renewable materials is contemplated as a substitute for petroleum-based product. Escherichia coli with advantages over other wild microorganisms, is considered as a powerful host for biofuels and chemicals. Here, we constructed a synthetic pathway of isoprene in E. coli by introducing an isoprene synthase (ispS) gene from Populus nigra, which catalyzes the conversion of dimethylallyl diphosphate (DMAPP) to isoprene. To improve the isoprene production, we overexpressed the native 1-deoxy-d-xylulose-5-phosphate (DXP) synthase gene (dxs) and DXP reductoisomerase gene (dxr) in E. coli, which catalyzed the first step and the second step of MEP pathway, respectively. The fed-batch fermentation results showed that overexpression of DXS is helpful for the improvement of isoprene production. Surprisingly, heterologous expression of dxs and dxr from Bacillus subtilis in the E. coli expressing ispS resulted in a 2.3-fold enhancement of isoprene production (from 94 to 314 mg/L). The promising results showed that dxs and dxr from B. subtilis functioned more efficiently on the enhancement of isoprene production than native ones. This could be caused by the consequence of great difference in protein structures of the two original DXSs. It could be practical to produce isoprene in E. coli via MEP pathway through metabolic engineering. This work provides an alternative way for production of isoprene by engineered E. coli via MEP pathway through metabolic engineering. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 生物基化学品 |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | NON-MEVALONATE PATHWAY ; 1-DEOXY-D-XYLULOSE 5-PHOSPHATE SYNTHASE ; SP STRAIN CL190 ; SWISS-MODEL ; TERPENOID BIOSYNTHESIS ; GENE ; PYRIDOXOL ; PRECURSOR ; DIPHOSPHATE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291034200008 |
| 公开日期 | 2012-11-07 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1110]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yaru,Yang, Jianming,Qin, Bo,et al. Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,90(6):1915-1922. |
| APA | Zhao, Yaru.,Yang, Jianming.,Qin, Bo.,Li, Yonghao.,Sun, Yuanzhang.,...&Xian, Mo.(2011).Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,90(6),1915-1922. |
| MLA | Zhao, Yaru,et al."Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 90.6(2011):1915-1922. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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