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Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography

文献类型：期刊论文

作者Feng, Dexin1; Li, Liangzhi1; Yang, Fang1; Tan, Weiqiang1; Zhao, Guoming1; Zou, Huibin1; Xian, Mo1; Zhang, Yingwei2
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2011-07-01
卷号91期号:2页码:399-405
关键词Ionic liquids Hydrophilic Recycle Alumina column chromatography Monosaccharide
中文摘要Pretreatment of cellulose with ionic liquids (ILs) can improve the efficiency of the hydrolysis by increasing the surface area of the substrates accessible to solvents and cellulases. However, the IL methods are facing challenges to separate the hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures. In this study, an alumina column chromatography (ACC) method was developed for the separation of hydrophilic IL N-methyl-N-methylimidazolium dimethyl phosphate ([Mmim][DMP]) and glucose, which was the main ingredient of the monosaccharide hydrolyzate. The processing parameters involved in ACC separation were investigated in detail. Our results showed that the recovery yields of [Mmim][DMP] and glucose can reach up to 93.38% and 90.14%, respectively, under the optimized parameters: the sampling ratio of 1:20 between the applied sample volume and the bed volume of the column; a gradient elution using methanol (100%, 150 ml) and then water (170 ml) as eluents with 1 ml/min flow rate. The recovered [Mmim][DMP] showed qualified property and was effective in a new hydrolysis reaction. In addition, scale-up ACC separations were successfully done with satisfied separation performance. The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the hydrolysis mixtures.
英文摘要Pretreatment of cellulose with ionic liquids (ILs) can improve the efficiency of the hydrolysis by increasing the surface area of the substrates accessible to solvents and cellulases. However, the IL methods are facing challenges to separate the hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures. In this study, an alumina column chromatography (ACC) method was developed for the separation of hydrophilic IL N-methyl-N-methylimidazolium dimethyl phosphate ([Mmim][DMP]) and glucose, which was the main ingredient of the monosaccharide hydrolyzate. The processing parameters involved in ACC separation were investigated in detail. Our results showed that the recovery yields of [Mmim][DMP] and glucose can reach up to 93.38% and 90.14%, respectively, under the optimized parameters: the sampling ratio of 1:20 between the applied sample volume and the bed volume of the column; a gradient elution using methanol (100%, 150 ml) and then water (170 ml) as eluents with 1 ml/min flow rate. The recovered [Mmim][DMP] showed qualified property and was effective in a new hydrolysis reaction. In addition, scale-up ACC separations were successfully done with satisfied separation performance. The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the hydrolysis mixtures.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题生物基化学品
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]IN-SITU SACCHARIFICATION ; AQUEOUS BIPHASIC SYSTEMS ; GREEN SOLVENTS ; WATER ; HYDROCARBONS ; PRETREATMENT ; ENHANCEMENT ; ADSORPTION ; EXTRACTION ; INTERFACE
收录类别SCI
语种英语
WOS记录号WOS:000292203200016
公开日期2012-11-07
源URL[http://ir.qibebt.ac.cn:8080/handle/337004/1111]  
专题青岛生物能源与过程研究所_材料生物技术研究中心
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China
2.Risun Coal Chem Grp Ltd, Xingtai, Peoples R China
推荐引用方式
GB/T 7714		 Feng, Dexin,Li, Liangzhi,Yang, Fang,et al. Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,91(2):399-405.
APA Feng, Dexin.,Li, Liangzhi.,Yang, Fang.,Tan, Weiqiang.,Zhao, Guoming.,...&Zhang, Yingwei.(2011).Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,91(2),399-405.
MLA Feng, Dexin,et al."Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 91.2(2011):399-405.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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