Production of phloroglucinol by Escherichia coli using a stationary-phase promoter
文献类型：期刊论文
| 作者 | Cao, Yujin1,2 ; Xian, Mo1
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 33期号:9页码:1853-1858 |
| 关键词 | Escherichia coli fic promoter phlD Phloroglucinol Plasmid copy number |
| 中文摘要 | Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher. |
| 英文摘要 | Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 生物基化学品 |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | GENES ; 2,4-DIACETYLPHLOROGLUCINOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293752700022 |
| 公开日期 | 2012-11-07 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1112]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Yujin,Xian, Mo. Production of phloroglucinol by Escherichia coli using a stationary-phase promoter[J]. BIOTECHNOLOGY LETTERS,2011,33(9):1853-1858. |
| APA | Cao, Yujin,&Xian, Mo.(2011).Production of phloroglucinol by Escherichia coli using a stationary-phase promoter.BIOTECHNOLOGY LETTERS,33(9),1853-1858. |
| MLA | Cao, Yujin,et al."Production of phloroglucinol by Escherichia coli using a stationary-phase promoter".BIOTECHNOLOGY LETTERS 33.9(2011):1853-1858. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。