Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet
文献类型：期刊论文
| 作者 | Shi YP(师彦平) ; Shi YP(师彦平); Chen J(陈娟)
|
| 刊名 | Acta Chromatographica
 |
| 出版日期 | 2009 |
| 卷号 | 21期号:2页码:341-354 |
| 关键词 | high-performance liquid chromatography traditional chinese medicine Qin-Bao-Hong antitussive tablet quality control |
| ISSN号 | 0231-2522 |
| 通讯作者 | 师彦平 |
| 中文摘要 | A high-performance liquid chromatographic (HPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous analysis of six active components (syringin, hyperoside, baicalin, quercetin, baicalein, and farrerol) of the Chinese medicinal preparation Qin-Bao-Hong antitussive tablet. The optimum conditions for separation were achieved on a 3.9 mm × 150 mm i.d., 5-μm particle, C18 column with a linear mobile phase gradient prepared from acetonitrile and 1% acetic acid at a flow rate of 1.0 mL min−1. Because of the different UV characteristics of these compounds, four detection wavelengths were used for the quantitative analysis (265 nm for syringin, 256 nm for hyperoside and quercetin, 277 nm for baicalin and baicalein, and 296 nm for farrerol). For all the analytes a good linear regression relationship (r > 0.999) was obtained between peak area and concentration over a relatively wide range. The method was validated for repeatability, precision, stability, accuracy, selectivity, and robustness. The validated method was successfully applied to simultaneous analysis of these active components in Qin-Bao-Hong antitussive tablet from different production batches. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the West Light Program of the Chinese Academy of Sciences (2007) |
| 语种 | 英语 |
| WOS记录号 | WOS:000267419700012 |
| 公开日期 | 2012-09-29 |
| 源URL | [http://210.77.64.217/handle/362003/1171]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Shi YP,Shi YP,Chen J. Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet[J]. Acta Chromatographica,2009,21(2):341-354. |
| APA | 师彦平,师彦平,&陈娟.(2009).Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet.Acta Chromatographica,21(2),341-354. |
| MLA | 师彦平,et al."Method development and validation for simultaneous HPLC analysis of six active components of the Chinese medicine Qin-Bao-Hong antitussive tablet".Acta Chromatographica 21.2(2009):341-354. |
入库方式： OAI收割
来源：兰州化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。