Determination of bergenin in rat urine, feces and tissues by RPHPLC method
文献类型：期刊论文
| 作者 | Shi YP(师彦平)
|
| 刊名 | J. Chin. Pharm. Sci.
 |
| 出版日期 | 2009 |
| 卷号 | 18页码:49-54 |
| 关键词 | RP-HPLC Bergenin Excretion Distribution |
| ISSN号 | 1003-1057 |
| 通讯作者 | 史彦斌 |
| 中文摘要 | A RP-HPLC analytical method.was developed.for determining bergenin.in rat urine, feces and.tissues. The separation.was performed on.a C18.analytical column with methanol–water as the mobile phase at a flow rate of 0.8 mL/min. The column.temperature.was 40 oC andthe eluate was monitored.at 220.nm. The.calibration.curve.for urine analysis.showed.a.good.linearity.(r = 0.9989) over the.range.of 0.5–100.μg/mL, and.the.absolute.recoveries were inthe range.of 90.0%–92.6%. A good.linearity.(r≥0.9996) for all tested.tissues was obtained.over the.range.of 0.3–150.μg/g except for lung, which had.a.linearity range.of 0.5–150.μg/g. The.absolute recoveries from tissues were higher than.63.9%. The.method was successfully applied to determine.the bergenin levels in several biological samples after rats were given bergenin formulation through tail vein injection. The results.demonstrated that the amount of bergenin recovered.from urine was.about 35.36% of the total dose, and.that from feces was less than 10%. Bergenin can be quickly and widely distributed in the rat, accumulating high levels in the kidney in comparison to all other tissues investigated. It was not distributed into the brain at any significant level owing to its hydrophilic property. |
| 学科主题 | 分析化学与药物化学 |
| 资助信息 | Natural Science Foundation (Grant No. ZS021-A25-051-N);Scientific & Technological Tackle Key Projects (Grant No. 2GS035-A43-048-01) of Gansu Province, China |
| 语种 | 英语 |
| 公开日期 | 2012-09-29 |
| 源URL | [http://210.77.64.217/handle/362003/1179]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 推荐引用方式 GB/T 7714 | Shi YP. Determination of bergenin in rat urine, feces and tissues by RPHPLC method[J]. J. Chin. Pharm. Sci.,2009,18:49-54. |
| APA | 师彦平.(2009).Determination of bergenin in rat urine, feces and tissues by RPHPLC method.J. Chin. Pharm. Sci.,18,49-54. |
| MLA | 师彦平."Determination of bergenin in rat urine, feces and tissues by RPHPLC method".J. Chin. Pharm. Sci. 18(2009):49-54. |
入库方式： OAI收割
来源：兰州化学物理研究所
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