High throughput screening of mutants of oat that are defective in triterpene synthesis
文献类型：期刊论文
| 作者 | Qin B(秦波)
|
| 刊名 | Phytochemistry
 |
| 出版日期 | 2010 |
| 卷号 | 71页码:1245-1252 |
| 关键词 | Avena strigosa Oats Cereals Mutants Triterpenes Sterols Plant defence |
| ISSN号 | 0031-9422 |
| 通讯作者 | Anne E.Osbourn |
| 中文摘要 | The triterpenes are a large and diverse group of plant natural products that have important functions in plant protection and food quality, and a range of pharmaceutical and other applications. Like sterols, they are synthesised from mevalonate via the isoprenoid pathway, the two pathways diverging after 2,3- oxidosqualene. During triterpene synthesis 2,3-oxidosqualene is cyclised to one of a number of potential products, the most common of these being the pentacyclic triterpene b-amyrin. Plants often produce complex mixtures of conjugated triterpene glycosides which may be derived from a single triterpene skeleton. The delineation, functional analysis and exploitation of triterpene pathways in plants therefore represent a substantial challenge. Here we have carried out high throughput screening to identify mutants of diploid oat (Avena strigosa) that are blocked in the early steps of triterpene synthesis. We also show that mutants that are affected in the first committed step in synthesis of b-amyrin-derived triterpenes, and so are unable to cyclise 2,3-oxidosqualene to b-amyrin (sad1 mutants), accumulate elevated levels of primary sterols. The major differences were in D-7-campesterol and D-7-avenasterol, which both increased several fold relative to wild-type levels. This is presumably due to accumulation of squalene and 2,3-oxidosqualene and consequent feedback into the sterol pathway, and is consistent with previous reports in which specific oxidosqualene cyclase inhibitors and elicitors of triterpene biosynthesis were shown to have inverse effects on the flux through the sterol and triterpene pathways. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | Dupont Agricultural Products (P.Mylona), the Gatsby Charitable Foundation (B. Qin and A. Osbourn), the Biotechnology and Biological Sciences Research Council (J. Eagles, F. Mellon, and A. Osbourn), and a Royal Society International Partnership Award (to L. Peña-Rodriguez and A. Osbourn) |
| 语种 | 英语 |
| 公开日期 | 2012-09-29 |
| 源URL | [http://210.77.64.217/handle/362003/1223]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 推荐引用方式 GB/T 7714 | Qin B. High throughput screening of mutants of oat that are defective in triterpene synthesis[J]. Phytochemistry,2010,71:1245-1252. |
| APA | 秦波.(2010).High throughput screening of mutants of oat that are defective in triterpene synthesis.Phytochemistry,71,1245-1252. |
| MLA | 秦波."High throughput screening of mutants of oat that are defective in triterpene synthesis".Phytochemistry 71(2010):1245-1252. |
入库方式： OAI收割
来源：兰州化学物理研究所
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