Simultaneous quantification of twelve active components in Yiqing granule by ultra performance liquid chromatography: application to quality control study
文献类型：期刊论文
| 作者 | Chen J(陈娟) ; Shi YP(师彦平); Shi YP(师彦平)
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| 刊名 | Biomed. Chromatogr.
 |
| 出版日期 | 2010 |
| 卷号 | 25页码:1043-1053 |
| 关键词 | ultra-performance liquid chromatography traditional Chinese medicine quality control yiqing granule protoberberine alkaloid |
| ISSN号 | 0269-3879 |
| 通讯作者 | 师彦平 |
| 中文摘要 | An ultra-performance liquid chromatography (UPLC) method coupled with photodiode array (PDA) detection has been developed and validated for simultaneous quantification of 12 active components (berberine, palmatine, jatrorrhizine, aloe-emodin, rhein, emodin, chrysophanol, physcion, baicalin, baicalein, wogonoside and wogonin) in Yiqing granule. Optimum separation were achieved on a C18 column (50 ￥ 2.1 mm i.d., 1.7 mm particle) through a 7.5 min gradient delivery of a mixture of A (acetonitrile) and B (0.1% aqueous phosphoric acid containing 1.8 mmol/L sodium dodecyl sulfonate and 10% acetonitrile, v/v) at a flow rate of 0.3 mL/min at 30°C. Because of the different UV characteristics of these components, three detection wavelengths were used for quantitative analysis. All of the analytes showed good linearity (r of >0.999). Themethod was validated for repeatability, precision, stability, accuracy and selectivity. The validated method was applied to quality control of Yiqing granule from different production batches. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the West Light Program of the Chinese Academy of Sciences (2007) |
| 语种 | 英语 |
| 公开日期 | 2012-10-08 |
| 源URL | [http://210.77.64.217/handle/362003/1258]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Chen J,Shi YP,Shi YP. Simultaneous quantification of twelve active components in Yiqing granule by ultra performance liquid chromatography: application to quality control study[J]. Biomed. Chromatogr.,2010,25:1043-1053. |
| APA | 陈娟,师彦平,&师彦平.(2010).Simultaneous quantification of twelve active components in Yiqing granule by ultra performance liquid chromatography: application to quality control study.Biomed. Chromatogr.,25,1043-1053. |
| MLA | 陈娟,et al."Simultaneous quantification of twelve active components in Yiqing granule by ultra performance liquid chromatography: application to quality control study".Biomed. Chromatogr. 25(2010):1043-1053. |
入库方式： OAI收割
来源：兰州化学物理研究所
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