Simultaneous determination of three diarylheptanoids and an a-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector.
文献类型：期刊论文
| 作者 | Liu JX(柳军玺) ; Di DL(邸多隆); Huang XY(黄新异)
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| 刊名 | Journal of Chromatogr-aphy A
 |
| 出版日期 | 2008 |
| 卷号 | 1190页码:80-85 |
| 关键词 | Juglans regia L. Diarylheptanoids α-Tetralone HPLC |
| ISSN号 | 0021-9673 |
| 通讯作者 | 邸多隆 |
| 中文摘要 | By optimizing extraction, separation and analytical conditions, a reliable and accurate high-performance liquid chromatographic (HPLC) method coupled with photodiode array detector (DAD) at room temperature is developed for simultaneous determination of three diarylheptanoids (juglanin A, juglanin B,rhoiptelol) and an _-tetralone derivative (regiolone) in methanol extracts from the green walnut husks (Juglans regia L.) The sample pretreatment process involved the reflux extraction using methanol as the extract with a ratio of liquor to sample of 15 mL/g. The separation was achieved on a SinoChrom ODS-AP C18 column with gradient elution using acetonitrile and 2% (v/v) acetic acid in water. The intra-day and inter-day precision (RSD%) for the analytes ranged from 1.08 to 1.51 and 0.60 to 1.13, respectively. The average recoveries obtained were from 88.4% to 96.2% for the analytes with RSDs below 3.13%. The correlation coefficients of the calibration curve exceeded 0.999. The detection limits were 0.51, 0.25, 0.32 and 0.35 ng at a signal-to-noise ratio of 3, respectively. Quantitative analyses of the samples from different grown sites and in obtained different months showed that the contents of the analytes varied significantly. The methodwas then successfully applied for the detection and isolation of a newdiarylheptanoid derivative in the green walnut husks (J. regia L.). The structure of the new compound was elucidated by various spectroscopic methods including 2DNMRtechniques (COSY, HMQC, HMBC), HR-ESI-MS and X-ray single-crystal diffraction analysis. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the “Hundred Talents Program” and “West Light Program” of Chinese Academy of Sciences; the National Natural Sciences Foundation of China (NSFC No.20775083). |
| 语种 | 英语 |
| 公开日期 | 2012-10-11 |
| 源URL | [http://210.77.64.217/handle/362003/1390]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Di DL(邸多隆) |
| 推荐引用方式 GB/T 7714 | Liu JX,Di DL,Huang XY. Simultaneous determination of three diarylheptanoids and an a-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector.[J]. Journal of Chromatogr-aphy A,2008,1190:80-85. |
| APA | 柳军玺,邸多隆,&黄新异.(2008).Simultaneous determination of three diarylheptanoids and an a-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector..Journal of Chromatogr-aphy A,1190,80-85. |
| MLA | 柳军玺,et al."Simultaneous determination of three diarylheptanoids and an a-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector.".Journal of Chromatogr-aphy A 1190(2008):80-85. |
入库方式： OAI收割
来源：兰州化学物理研究所
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