Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte
文献类型：期刊论文
| 作者 | Zhao L(赵亮) ; Jiang SX(蒋生祥); Liu X(刘霞); Jiang SX(蒋生祥)
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| 刊名 | Journal of Pharmaceutical and Biomedical Analysis
 |
| 出版日期 | 2007 |
| 卷号 | 44页码:881–886 |
| 关键词 | Flavonoid Capillary electrophoresis Lamiophlomis rotata Association constant |
| ISSN号 | 0731-7085 |
| 通讯作者 | 蒋生祥 |
| 中文摘要 | A capillary electrophoresis methodwas developed for the simultaneous determination of five flavonoids such as luteolin-7-O-glucoside, isorhamnetin, apigenin, luteolin and quercetin in Lamiophlomis rotata (Benth.) Kudo. Optimal conditions were obtained at pH 9.0 with 30mM borate as buffer containing 8% (v/v) acetonitrile, 20 kV as driving voltage and 210 nm as detection wavelength. The association constant K and the change in Gibbs free energy ( G) of the interaction of flavonoids with borate anion ion (a typical ion-dipole or ion-induced dipole interaction) were calculated for the quantitative evaluation and characterization of the interaction. The described method was successfully applied for the rapid and efficient quality control by quantifying flavonoids in L. rotata. Repeatability tests showed that the R.S.D.s of both intra- and inter-day migration times and peak areas were less than 5%. The LOD of the five flavonoids was less than 3.75 mg/l. Recovery results ranged from 94.2% to 105.1%. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the Major Frontier Projects of the Chinese Academy of Sciences (No. KGCX2-213-08);the West Light Program of China (2003);the Young-Aged Scientific Foundation of Gansu Province (No. 3ZS041-A25-23) |
| 语种 | 英语 |
| WOS记录号 | WOS:000249182100007 |
| 公开日期 | 2012-10-11 |
| 源URL | [http://210.77.64.217/handle/362003/1410]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Jiang SX(蒋生祥); Jiang SX(蒋生祥) |
| 推荐引用方式 GB/T 7714 | Zhao L,Jiang SX,Liu X,et al. Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte[J]. Journal of Pharmaceutical and Biomedical Analysis,2007,44:881–886. |
| APA | 赵亮,蒋生祥,刘霞,&蒋生祥.(2007).Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte.Journal of Pharmaceutical and Biomedical Analysis,44,881–886. |
| MLA | 赵亮,et al."Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte".Journal of Pharmaceutical and Biomedical Analysis 44(2007):881–886. |
入库方式： OAI收割
来源：兰州化学物理研究所
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