Determination of free isomeric oleanolic acid and ursolic acid in Pterocephalus hookeri by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | Jiang SX(蒋生祥) ; Liu X(刘霞); Liu X(刘霞)
|
| 刊名 | J Pharm Biomed Anal
 |
| 出版日期 | 2007 |
| 卷号 | 43页码:1331-1334 |
| 关键词 | Capillary zone electophoresis Pterocephalus hookeri Oleanolic acid Ursolic acid b-Cyclodextrin |
| ISSN号 | 0731-7085 |
| 通讯作者 | 刘霞 |
| 中文摘要 | A rapid capillary zone electophoresis (CZE) method for the quantification of two bioactive terpenes in Pterocephalus hookeri was developed. With b-cyclodextrin as an additive, excellent resolution for these hydrophobic isomers can be obtained in less than 11 min. Linear calibration range for oleanolic acid was between 15.6 and 1000 g/ml (r = 0.9998), for ursolic acid between 31.2 and 1000 g/ml (r = 0.9992). The limits of detection for oleanolic acid and ursolic acid were 3.4 and 3.8 g/ml, respectively. The contents of the free oleanolic acid and ursolic acid in P. hookeri were determined with recoveries ranging from 95.2 to 106.0%. The contents of oleanolic and ursolic acids were found to be 0.21 mg/g (RSD 5.49%) and 0.53 mg/g (RSD 3.37%), respectively. After hydrolysis by acid, these values were 1.12 mg/g (RSD 3.29%) and 0.43 mg/g (RSD 3.42%). |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the Scientific Foundation of Chinese Academy of Science (no. KGCX2-SW-213-08) |
| 语种 | 英语 |
| WOS记录号 | WOS:000245262300019 |
| 公开日期 | 2012-10-11 |
| 源URL | [http://210.77.64.217/handle/362003/1421]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Liu X(刘霞); Liu X(刘霞) |
| 推荐引用方式 GB/T 7714 | Jiang SX,Liu X,Liu X. Determination of free isomeric oleanolic acid and ursolic acid in Pterocephalus hookeri by capillary zone electrophoresis[J]. J Pharm Biomed Anal,2007,43:1331-1334. |
| APA | 蒋生祥,刘霞,&刘霞.(2007).Determination of free isomeric oleanolic acid and ursolic acid in Pterocephalus hookeri by capillary zone electrophoresis.J Pharm Biomed Anal,43,1331-1334. |
| MLA | 蒋生祥,et al."Determination of free isomeric oleanolic acid and ursolic acid in Pterocephalus hookeri by capillary zone electrophoresis".J Pharm Biomed Anal 43(2007):1331-1334. |
入库方式： OAI收割
来源：兰州化学物理研究所
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