Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography
文献类型：期刊论文
| 作者 | Shi YP(师彦平) ; Shi YP(师彦平)
|
| 刊名 | Analytica Chimica Acta
 |
| 出版日期 | 2006 |
| 卷号 | 561页码:119-125 |
| 关键词 | Microemulsion electrokinetic chromatography Aristolochic acids Chinese herbs nephropathy |
| ISSN号 | 0003-2670 |
| 通讯作者 | 师彦平 |
| 中文摘要 | A microemulsion electrokinetic chromatography (MEEKC) method has been firstly developed and validated for the separation of aristolochic acids A, B, C and D, 7-hydroxy-aristolochic acid A and aristolic acid in herbal medicines. With the optimized experimental conditions (octane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer 89.27% (w/w), with 15 kV as applied voltage, pH 9.2, 25℃ as capillary temperature), all of the six analytes were baseline-separated (any resolution was over 2) in peaks of good shapes within 16 min. Aristolochic acids A, B, C and aristolic acid were quantitative determined and acceptable precision (intra-day repeatability (n = 6) and inter-day reproducibility (n = 3) of the method in terms of migration times were within 0.98 and 2.75% R.S.D. in terms of migration times and within 5.09 and 5.93% R.S.D. in terms of peak areas, respectively) and linearity (r > 0.995) and recovery (ranged between 97 and 104%) were obtained. Moreover, the method was successfully validated and applied to the determination of four analytes in herbal medicines. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the “Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000;National Natural Science Foundation of China (NSFC No. 20475057) |
| 语种 | 英语 |
| WOS记录号 | WOS:000235945200016 |
| 公开日期 | 2012-10-14 |
| 源URL | [http://210.77.64.217/handle/362003/1447]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Shi YP,Shi YP. Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography[J]. Analytica Chimica Acta,2006,561:119-125. |
| APA | 师彦平,&师彦平.(2006).Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography.Analytica Chimica Acta,561,119-125. |
| MLA | 师彦平,et al."Separation and determination of aristolochic acids in herbal medicines by microemulsion electrokinetic chromatography".Analytica Chimica Acta 561(2006):119-125. |
入库方式： OAI收割
来源：兰州化学物理研究所
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