Determination and pharmacokinetic study of bergenin in rat piasma by RP-HPLC method
文献类型：期刊论文
| 作者 | Shi YP(师彦平) ; Shi YP(师彦平)
|
| 刊名 | Biomed. Chromatogr.
 |
| 出版日期 | 2006 |
| 卷号 | 20页码:1065-1070 |
| ISSN号 | 0269-3879 |
| 关键词 | bergenin RP-HPLC pharmacokinetics |
| 通讯作者 | 师彦平 |
| 中文摘要 | A validated reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the determination of bergenin in rat plasma. Bergenin in rat plasma was extracted with methanol, which also acted as a deproteinization agent. Chromatographic separation of bergenin was performed on a C18 column, with a mobile phase of methanol–water (22:78, v/v) at a flow-rate of 0.8 mL/min and an operating temperature of 40℃, and UV detection was set at 220 nm. The calibration curve was linear over the range 0.25–50 μg/mL (r = 0.9990) in rat plasma. The limit of quantification was 0.25 μg/mL using a plasma sample of 100 μL. The extraction recoveries were 83.40 ± 6.02, 81.49 ± 2.40 and 72.51 ± 2.64% at concentrations of 0.5, 5 and 50 μg/mL, respectively. The intra-day and inter-day precision and accuracy were validated by relative standard deviation (RSD%) and relative error (RE%), which were in the ranges 3.74–9.91 and −1.6–8.0%. After intravenous administration to rats at the dose of 11.25 mg/kg, the plasma concentration–time curve of bergenin was best conformed to a two-compartment open model. The main pharmacokinetic parameters indicated that bergenin exhibited a wide distribution and moderate elimination velocity in rat. |
| 学科主题 | 分析化学与药物化学 |
| 资助信息 | Natural Science Foundation (ZS021-A25-051-N), Gansu province, China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-10-14 |
| 源URL | [http://210.77.64.217/handle/362003/1450]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Shi YP,Shi YP. Determination and pharmacokinetic study of bergenin in rat piasma by RP-HPLC method[J]. Biomed. Chromatogr.,2006,20:1065-1070. |
| APA | 师彦平,&师彦平.(2006).Determination and pharmacokinetic study of bergenin in rat piasma by RP-HPLC method.Biomed. Chromatogr.,20,1065-1070. |
| MLA | 师彦平,et al."Determination and pharmacokinetic study of bergenin in rat piasma by RP-HPLC method".Biomed. Chromatogr. 20(2006):1065-1070. |
入库方式： OAI收割
来源：兰州化学物理研究所
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