Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography
文献类型：期刊论文
| 作者 | Zhao YF(赵艳芳) ; Luo XP(罗兴平) ; Ming YF(明永飞) ; Chen LR(陈立仁) ; Li YM(李永民) |
| 刊名 | Journal of Pharmaceutical and Biomedical Analysis
 |
| 出版日期 | 2006 |
| 卷号 | 41页码:124-128 |
| 关键词 | Micellar electrokinetic chromatography b-Cyclodextrin Resibufogenin Cinobufagin Toad venom |
| ISSN号 | 0731-7085 |
| 通讯作者 | 陈立仁 |
| 中文摘要 | A rapid cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method was proposed for the determination of resibufogenin and cinobufagin in the Chinese herbal extracts from toad venom and its medicinal preparation (Liushen tablet). The two components have the close structural similarity and similar hydrophobicity, which result in poor resolution in normal MEKC. The addition of neutral b- CD to the MEKC system was found to improve the separation of the studied compounds. The effects of several CD-MEKC parameters on the resolutions were evaluated systematically. Based on the investigation, a background electrolyte solution consisting of 10mM borate buffer adjusted to pH 8.5, 40mM sodium dodecyl sulfate (SDS), 12mM -CD and 10% (v/v) of methanol was found to be optimal conditions for the fast separation. The contents of resibufogenin and cinobufagin were successfully determined within 5 min, with satisfactory repeatability and recovery. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the Foundation for “Bairen Jihua” of CAS in 2001; National Natural Science Foundation of China (no. 20375045) |
| 语种 | 英语 |
| 公开日期 | 2012-10-14 |
| 源URL | [http://210.77.64.217/handle/362003/1459]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao YF,Luo XP,Ming YF,et al. Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,41:124-128. |
| APA | 赵艳芳,罗兴平,明永飞,陈立仁,&李永民.(2006).Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography.Journal of Pharmaceutical and Biomedical Analysis,41,124-128. |
| MLA | 赵艳芳,et al."Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography".Journal of Pharmaceutical and Biomedical Analysis 41(2006):124-128. |
入库方式： OAI收割
来源：兰州化学物理研究所
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