Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression
文献类型：期刊论文
| 作者 | Zhao, Xi ; Chao, Yong-Lie ; Wan, Qian-Bing ; Chen, Xin-Min ; Su, Peng ; Sun, Jian ; Tang, Yaxiong |
| 刊名 | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 89期号:12页码:875-882 |
| 关键词 | flavokawain B (FKB) apoptosis Bcl-2 Bim ACC-2 |
| ISSN号 | 0008-4212 |
| 产权排序 | 2 |
| 通讯作者 | Tang, YX ; Sun, Jian |
| 英文摘要 | Novel effective drugs are still urgently needed in the prevention and treatment of oral adenoid cystic carcinoma (ACC). In this study, we have assessed the antitumor potential and molecular mechanisms of flavokawain B (FKB) as a kava chalcone on the ACC-2 cell line in vitro. The results demonstrated that FKB could significantly inhibit the cell proliferation of ACC-2 in a dose-dependent manner that was associated with induced apoptosis and cell cycle G2-M arrest, and the half maximal inhibitory concentration (IC(50)) of flavokawain-B treatment for 48 h was estimated to be 4.69 +/- 0.43 mu mol/L. Mechanistically, FKB could induce the release of cytochrome c from mitochondria into the cytosol, and activate the cleavage of caspase-3 and, eventually, the poly(ADP-ribose) polymerase (PARP), in a dose-dependent manner, leading to marked apoptotic effect of ACC-2 cells. The apoptotic action of FKB was associated with the increased expression of proapoptotic proteins: Bim, Bax, Bak and a decreased expression of antiapoptotic Bcl-2. Among them, Bim expression was significantly induced by FKB, and knockdown of Bim expression by short-hairpin RNAs attenuated the inhibitory effect induced by FKB on ACC-2 cells. These results suggest Bim may be one of the potential transcriptional targets, and suggests the potential usefulness of FKB for the prevention and treatment of ACC. |
| 学科主题 | Pharmacology & Pharmacy; Physiology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[81072662, 91013006] |
| 语种 | 英语 |
| WOS记录号 | WOS:000298441800002 |
| 公开日期 | 2012-11-09 |
| 源URL | [http://210.75.237.14/handle/351003/23518]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhao, Xi,Chao, Yong-Lie,Wan, Qian-Bing,et al. Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression[J]. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,2011,89(12):875-882. |
| APA | Zhao, Xi.,Chao, Yong-Lie.,Wan, Qian-Bing.,Chen, Xin-Min.,Su, Peng.,...&Tang, Yaxiong.(2011).Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression.CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,89(12),875-882. |
| MLA | Zhao, Xi,et al."Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression".CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 89.12(2011):875-882. |
入库方式： OAI收割
来源：成都生物研究所
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