A high-sensitivity UPLC-MS/MS method for simultaneous determination and confirmation of triptolide in zebrafish embryos
文献类型：期刊论文
| 作者 | Zhou, Yan ; He, Ming-Fang ; Choi, Franky Fung-Kei ; He, Zhi-Heng ; Song, Jing-Zheng ; Qiao, Chun-Feng ; Li, Song-Lin ; Xu, Hong-Xi |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2011 |
| 卷号 | 25期号:8页码:851-857 |
| 关键词 | triptolide zebra-fish UPLC/MS/MS MRM PIC |
| ISSN号 | 0269-3879 |
| 产权排序 | 1 |
| 通讯作者 | Xu, HX (reprint author), Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China. |
| 英文摘要 | A high-sensitivity ultra-performance liquid-chromatography (UPLC) coupled with tandem mass spectrometric method was developed for simultaneous quantification and confirmation of triptolide in both zebrafish embryos and the aqueous-exposure solution on a tandem quadrupole mass spectrometer (TQ-MS). This was achieved by performing quantification using the multiple reaction monitoring (MRM) acquisition with simultaneous characterization of the MRM peak using product ion confirmation (PIC) acquisition as it elutes from the chromatographic system. Separation was achieved on a 1.7 mu m C(18) UPLC column using 0.1% formic acid water-acetonitrile mobile phase with a cycle time of 6 min. The linear range of 0.115-360 ng/mL, and lower limits of detection of 0.02 ng/mL and quantification of 0.064 ng/mL were established. This method was successfully applied to determine the time course of triptolide absorption by zebrafish embryos and the amount of triptolide remaining in the culture medium after administration of two triptolide dosages at three time points. This coupled MRM with PIC approach could provide both qualitative and quantitative results without the need for repetitive analyses. This resulted in the reduction of further confirmative experiments and analytical time, and ultimately increased laboratory productivity. Copyright (C) 2010 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Jockey Club Charities Trust |
| 语种 | 英语 |
| WOS记录号 | WOS:000292601500001 |
| 公开日期 | 2012-11-09 |
| 源URL | [http://210.75.237.14/handle/351003/23535]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhou, Yan,He, Ming-Fang,Choi, Franky Fung-Kei,et al. A high-sensitivity UPLC-MS/MS method for simultaneous determination and confirmation of triptolide in zebrafish embryos[J]. BIOMEDICAL CHROMATOGRAPHY,2011,25(8):851-857. |
| APA | Zhou, Yan.,He, Ming-Fang.,Choi, Franky Fung-Kei.,He, Zhi-Heng.,Song, Jing-Zheng.,...&Xu, Hong-Xi.(2011).A high-sensitivity UPLC-MS/MS method for simultaneous determination and confirmation of triptolide in zebrafish embryos.BIOMEDICAL CHROMATOGRAPHY,25(8),851-857. |
| MLA | Zhou, Yan,et al."A high-sensitivity UPLC-MS/MS method for simultaneous determination and confirmation of triptolide in zebrafish embryos".BIOMEDICAL CHROMATOGRAPHY 25.8(2011):851-857. |
入库方式： OAI收割
来源：成都生物研究所
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