Antiosteoporotic compounds from seeds of Cuscuta chinensis
文献类型：期刊论文
| 作者 | Yang, Lijuan ; Chen, Qianfeng ; Wang, Fei ; Zhang, Guolin |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 135期号:2页码:553-560 |
| 关键词 | Cuscuta chinensis Alkaline phosphatase Flavonoid Osteoblast Estrogen receptor |
| ISSN号 | 0378-8741 |
| 产权排序 | 1 |
| 通讯作者 | Wang, Fei ; Zhang, Guolin |
| 英文摘要 | Ethnopharmacological relevance: The seeds of Cuscuta chinensis (Tu-Si-Zi, TSZ) have long been used for the treatment of osteoporosis in China and some Asian countries. The compounds in TSZ responsible for the antiosteoporotic activity are still poorly understood. Aim of the study: The present study was designed to investigate the osteogenic compounds in TSZ, and to evaluate their antiosteoporotic effects in osteoblastic cells. Materials and methods: Osteoblast-like UMR-106 cells were used for bioactivity-guided isolation of the active compounds. The activity of alkaline phosphatase (ALP) in UMR-106 cells was measured by p-nitrophenyl sodium phosphate assay. The proliferation of UMR-106 cells was assayed by Alamar-Blue method. Estrogenic activity of the extracts and isolated compounds was evaluated by activation of estrogen response element (ERE) luciferase reporter expression in Hela cells co-transfected with human estrogen receptor subtypes (ER alpha or ER beta) expression vectors and 5 x ERE luciferase reporter plasmid. Antiestrogenic activity of the extracts and isolated compounds were evaluated by activation of activator protein-1 (AP-1) luciferase reporter expression in HeLa cells co-transfected with human estrogen receptor subtypes (ER alpha or ER beta) expression vectors and 6 x AP-1 luciferase reporter plasmid. Results: ALP-guided fractionation led to the isolation of five known flavonoids, quercetin, kaempferol, isorhamnetin, hyperoside and astragalin from the crude ethanolic extract of TSZ. Further study showed that kaempferol and hyperoside significantly increased the ALP activity in UMR-106 cells. Astragalin promoted the proliferation of UMR-106 cells whereas other compounds had no such effect. The isolated compounds showed estrogenic activity but quercetin, kaempferol and isorhamnetin showed more potent ERP agonist activity. However, compared with their ER agonist activity, only quercetin and kaempferol showed potent ER antagonist activity by activating ER alpha/beta-mediated AP-1 reporter expression. Conclusions: Our findings validated the clinical use of TSZ in the treatment of osteoporosis, and demonstrated that kaempferol and hyperoside are the active compounds in TSZ for the osteogenic effect. (C) 2011 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences[KSCX2-YW-R-078]; West Light Foundation; National Natural Science Foundation of China[20932007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000291716000045 |
| 公开日期 | 2012-11-09 |
| 源URL | [http://210.75.237.14/handle/351003/23548]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Yang, Lijuan,Chen, Qianfeng,Wang, Fei,et al. Antiosteoporotic compounds from seeds of Cuscuta chinensis[J]. JOURNAL OF ETHNOPHARMACOLOGY,2011,135(2):553-560. |
| APA | Yang, Lijuan,Chen, Qianfeng,Wang, Fei,&Zhang, Guolin.(2011).Antiosteoporotic compounds from seeds of Cuscuta chinensis.JOURNAL OF ETHNOPHARMACOLOGY,135(2),553-560. |
| MLA | Yang, Lijuan,et al."Antiosteoporotic compounds from seeds of Cuscuta chinensis".JOURNAL OF ETHNOPHARMACOLOGY 135.2(2011):553-560. |
入库方式： OAI收割
来源：成都生物研究所
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