中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver

文献类型:期刊论文

作者Shujun Zhang
刊名J Ind Microbiol Biotechnol
出版日期2010
卷号37期号:1页码:447-454
中文摘要

Eucommia ulmoides Oliver is a traditional medicinal plant of China, and it is one of the main sources of chlorogenic acid. Chlorogenic acid is an ester of caffeic acid, quinic acid, and a phenolic compound that has antibacterial, antifungal, antioxidant, and antitumor activities. The purpose of this study was to determine whether endophytic fungi isolated from Eucommia ulmoides Oliver had the same ability to produce chlorogenic acid. Primary screening was done by antibacterial and antifungal reactions, and the strain reselection was done with high-performance liquid chromatography (HPLC) to identify the fermentation products of the selected strains. Extracts of the leaf and cortex of Eucommia ulmoides Oliver were also deteted by HPLC, then positive results of HPLC were analyzed by GC-MS and LC-MS. In this study, 29 strains were isolated from Eucommia ulmoides Oliver. Most of them had antibacterial activity, and a few of them had antifungal activity. One ingredient of the B5 extract had a retention time identical to that of authentic chlorogenic acid. With GC-MS, other ingredients, isocoumarin and p-chlorocinnamide, were found. With LC-MS, chlorogenic acid and geniposide related to Eucommia ulmoides Oliver were found. The strain B5 was identified as Sordariomycete sp. Thus, endophytic fungi may produce the bioactive compound chlorogenic acid, as their host plant does, and could be used for the production of chlorogenic acid by fermentation in the future.


学科主题仿真与模拟
语种英语
公开日期2012-11-12
源URL[http://ir.qibebt.ac.cn:8080/handle/337004/1349]  
专题青岛生物能源与过程研究所_仿真模拟团队
推荐引用方式
GB/T 7714
Shujun Zhang. Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver[J]. J Ind Microbiol Biotechnol,2010,37(1):447-454.
APA Shujun Zhang.(2010).Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver.J Ind Microbiol Biotechnol,37(1),447-454.
MLA Shujun Zhang."Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver".J Ind Microbiol Biotechnol 37.1(2010):447-454.

入库方式: OAI收割

来源:青岛生物能源与过程研究所

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