Isolation and characterization of a chromium-resistant bacterium Serratia sp. Cr-10 from a chromate–contaminated site.
文献类型：期刊论文
| 作者 | Zhang KD., Li FL. 2011. |
| 刊名 | Appl Microbiol Biotechnol
 |
| 出版日期 | 2011-05 |
| 卷号 | 90期号:3页码:1163-1169 |
| 关键词 | Cr(VI) reduction – Serratia sp. – Batch reduction – Bioremediation |
| 中文摘要 | A novel bacterium, Cr-10, was isolated from a chromium-contaminated site and capable of removing toxic chromium species from solution by reducing hexavalent chromium to an insoluble precipitate. Sequence analysis of 16S rRNA gene of strain Cr-10 showed that it was most closely related to Serratia rubidaea JCM 1240T (97.68%). Physiological and chemotaxonomic data also supported that strain Cr-10 was identified asSerratia sp., a genus which was never specially reported chromate-resistant before. Serratia sp., Cr-10 was tolerant to a concentration of 1,500 mg Cr(VI) L−1, which was the highest level reported until now. The optimum pH and temperature for reduction of Cr(VI) by Serratia sp. Cr-10 were found to be 7.0 and 37 °C, respectively. The Cr(VI) reduction was significantly influenced by additional carbon sources, and among them fructose and lactose offered maximum reduction, with a rate of 0.28 and 0.25 mg Cr(VI) L−1 h−1, respectively. The cell-free extracts and filtrate of the culture were able to reduce Cr(VI) while concentration of total chromium remained stable in the process, indicating that the enzyme-catalyzed mechanism was applied in Cr(VI) reduction by the isolate. Additionally, it was found that there was hardly any chromium on the cell surface of the strain, further supporting that reduction, rather than bioadsorption, plays a major role in the Cr(VI) removal. |
| 学科主题 | 微生物资源 |
| 公开日期 | 2012-11-11 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1243]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 推荐引用方式 GB/T 7714 | Zhang KD., Li FL. 2011.. Isolation and characterization of a chromium-resistant bacterium Serratia sp. Cr-10 from a chromate–contaminated site.[J]. Appl Microbiol Biotechnol,2011,90(3):1163-1169. |
| APA | Zhang KD., Li FL. 2011..(2011).Isolation and characterization of a chromium-resistant bacterium Serratia sp. Cr-10 from a chromate–contaminated site..Appl Microbiol Biotechnol,90(3),1163-1169. |
| MLA | Zhang KD., Li FL. 2011.."Isolation and characterization of a chromium-resistant bacterium Serratia sp. Cr-10 from a chromate–contaminated site.".Appl Microbiol Biotechnol 90.3(2011):1163-1169. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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