氧化葡萄糖杆菌合成右旋糖酐糊精酶的pH两阶段控制策略
文献类型:期刊论文
作者 | 王舒, 毛相朝, 李福利, 魏东芝. 2011. . | |||
刊名 | 微生物学通报
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出版日期 | 2011 | |||
卷号 | 38期号:8页码:1155~1159 | |||
中文摘要 |
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英文摘要 | In order to enhance the yield of dextran dextrinase (DDase) produced by Gluconobacter oxydans DSM 2003, the effects of pH from 3.5 to 6 on cell growth and DDase activity were investigated in a 3 L fermentor. Based on time courses of cell growth and DDase activity, we developed a two-stage pH control strategy, in which pH was controlled at 5.0 for the first 6 h and then 4.0 for remaining time. Under the optimized strategy, the production of DDase had a significant improvement. Moreover, the maximal DDase activity reached 4.03 U/mL, 38.5% and 1 147% more than that from the strategy without pH control and in the 250 mL shake flasks, respectively. Meanwhile, the fermentation time of DDase in the fermentor was also shortened from 47 h to 15 h compared to that in 250 mL shake flasks. | |||
学科主题 | 微生物资源 | |||
收录类别 | 其他 | |||
语种 | 中文 | |||
公开日期 | 2012-11-11 | |||
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1245] ![]() | |||
专题 | 青岛生物能源与过程研究所_微生物资源团队 | |||
推荐引用方式 GB/T 7714 | 王舒, 毛相朝, 李福利, 魏东芝. 2011. .. 氧化葡萄糖杆菌合成右旋糖酐糊精酶的pH两阶段控制策略[J]. 微生物学通报,2011,38(8):1155~1159. | |||
APA | 王舒, 毛相朝, 李福利, 魏东芝. 2011. ..(2011).氧化葡萄糖杆菌合成右旋糖酐糊精酶的pH两阶段控制策略.微生物学通报,38(8),1155~1159. | |||
MLA | 王舒, 毛相朝, 李福利, 魏东芝. 2011. .."氧化葡萄糖杆菌合成右旋糖酐糊精酶的pH两阶段控制策略".微生物学通报 38.8(2011):1155~1159. |
入库方式: OAI收割
来源:青岛生物能源与过程研究所
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