Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri
文献类型：期刊论文
| 作者 | Li FL. ; H. Hinderberger ; H. Seedorf ; J. Zhang ; W. Buckel ; R. K. Thauer. |
| 刊名 | J. Bacteriol
 |
| 出版日期 | 2008 |
| 卷号 | 190期号:1页码:843-850 |
| 中文摘要 | Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously observed is due to the fact that the cell extracts catalyze the reduction of acetyl-CoA with NADH via crotonyl-CoA to butyryl-CoA. The cytoplasmic butyryl-CoA dehydrogenase complex was purified and is shown to couple the endergonic reduction of ferredoxin (E0' = -410 mV) with NADH (E0' = -320 mV) to the exergonic reduction of crotonyl-CoA to butyryl-CoA (E0' = -10 mV) with NADH. The stoichiometry of the fully coupled reaction is extrapolated to be as follows: 2 NADH + 1 oxidized ferredoxin + 1 crotonyl-CoA = 2 NAD+ + 1 ferredoxin reduced by two electrons + 1 butyryl-CoA. The implications of this finding for the energy metabolism of butyrate-forming anaerobes are discussed in the accompanying paper. |
| 学科主题 | 微生物资源 |
| 语种 | 英语 |
| 公开日期 | 2012-11-11 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1252]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 推荐引用方式 GB/T 7714 | Li FL.,H. Hinderberger,H. Seedorf,et al. Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri[J]. J. Bacteriol,2008,190(1):843-850. |
| APA | Li FL.,H. Hinderberger,H. Seedorf,J. Zhang,W. Buckel,&R. K. Thauer..(2008).Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri.J. Bacteriol,190(1),843-850. |
| MLA | Li FL.,et al."Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri".J. Bacteriol 190.1(2008):843-850. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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