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Phagemid Vectors for Phage Display: Properties, Characteristics and Construction
文献类型:期刊论文
作者 | Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2 |
刊名 | JOURNAL OF MOLECULAR BIOLOGY
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出版日期 | 2012-03-30 |
卷号 | 417期号:3页码:129-143 |
关键词 | phage display phagemid filamentous phage phagemid vector construction |
中文摘要 | Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. |
英文摘要 | Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | 生物传感器技术 |
类目[WOS] | Biochemistry & Molecular Biology |
研究领域[WOS] | Biochemistry & Molecular Biology |
关键词[WOS] | COMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000301805800001 |
公开日期 | 2012-11-10 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1210] ![]() |
专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
作者单位 | 1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China 4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China |
推荐引用方式 GB/T 7714 | Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143. |
APA | Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,Petrenko, Valery,&Liu, Aihua.(2012).Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.JOURNAL OF MOLECULAR BIOLOGY,417(3),129-143. |
MLA | Qi, Huan,et al."Phagemid Vectors for Phage Display: Properties, Characteristics and Construction".JOURNAL OF MOLECULAR BIOLOGY 417.3(2012):129-143. |
入库方式: OAI收割
来源:青岛生物能源与过程研究所
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