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Phagemid Vectors for Phage Display: Properties, Characteristics and Construction

文献类型:期刊论文

作者Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2
刊名JOURNAL OF MOLECULAR BIOLOGY
出版日期2012-03-30
卷号417期号:3页码:129-143
关键词phage display phagemid filamentous phage phagemid vector construction
中文摘要Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.
英文摘要Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题生物传感器技术
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]COMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION
收录类别SCI
语种英语
WOS记录号WOS:000301805800001
公开日期2012-11-10
源URL[http://ir.qibebt.ac.cn:8080/handle/337004/1210]  
专题青岛生物能源与过程研究所_生物传感技术团队
作者单位1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
推荐引用方式
GB/T 7714
Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143.
APA Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,Petrenko, Valery,&Liu, Aihua.(2012).Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.JOURNAL OF MOLECULAR BIOLOGY,417(3),129-143.
MLA Qi, Huan,et al."Phagemid Vectors for Phage Display: Properties, Characteristics and Construction".JOURNAL OF MOLECULAR BIOLOGY 417.3(2012):129-143.

入库方式: OAI收割

来源:青岛生物能源与过程研究所

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