Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection
文献类型：期刊论文
| 作者 | Liu, Aihua1,2,3; Wu, Liyou1; He, Zhili1; Zhou, Jizhong1 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 401期号:6页码:2003-2011 |
| 关键词 | DNA microarray Sensitivity Silica nanoparticles chemically doped dye Biotin-streptavidin interaction Reverse microemulsion reaction |
| 中文摘要 | Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins. |
| 英文摘要 | Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | 生物传感器技术 |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SINGLE-NUCLEOTIDE POLYMORPHISM ; RESONANCE ENERGY-TRANSFER ; REVERSE MICROEMULSION ; GOLD NANOPARTICLES ; URIC-ACID ; BIOSENSORS ; NANOTUBES ; GEOCHIP ; PROBES ; HYBRIDIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295510100027 |
| 公开日期 | 2012-11-10 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1214]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Aihua,Wu, Liyou,He, Zhili,et al. Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,401(6):2003-2011. |
| APA | Liu, Aihua,Wu, Liyou,He, Zhili,&Zhou, Jizhong.(2011).Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,401(6),2003-2011. |
| MLA | Liu, Aihua,et al."Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401.6(2011):2003-2011. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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