Abstract

INTRODUCTION:

The suitability of the application of ultrafiltration (UF) to harvest Chlorella sp. from the culture medium was examined. We investigated the effects of two improved UF system, forward air-water flushing and backwash with permeate, on the concentration process.

MATERIALS AND METHODS:

Backwash with permeate was selected as an optimization of the improved UF system, which was more effective for permeate flux recovery. Moreover, the hollow fiber UF system by adding periodical backwash with permeate was examined for Chlorella sp. harvesting.

RESULTS AND DISCUSSION:

It was found that Chlorella sp. could be concentrated with high recovery in a lab-scale experiment. An overall algal biomass recovery of above 90% was achieved when the volume concentration factor was 10. For an original biomass of 1.3 ± 0.05 g/L, 1 min backwash followed by 20 min forward concentrating was more effective, which resulted in a recovery of 94% and a high average flux of 30.3 L/m(2)/h. In addition, the algal recovery was highly correlated to the volume concentration factor and the initial biomass. A high concentration factor or a high initial biomass resulted in a low biomass recovery.