中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments

文献类型:期刊论文

作者Xu, Meiying1,4,5; Chen, Xingjuan1,4,5; Qiu, Mengde1; Zeng, Xiaowei2,3; Xu, Jian2,3; Deng, Daiyong1,4,5; Sun, Guoping1,4,5; Li, Xiang6; Guo, Jun1,4,5
刊名PLOS ONE
出版日期2012-01-25
卷号7期号:1页码:e30439
中文摘要
Polybrominated diphenyl ethers (PBDEs) can be reductively degraded by microorganisms under anaerobic conditions. However, little is known about the effect of electron donors on microbial communities involved in PBDEs degradation. Here we employed 454 Titanium pyrosequencing to examine the phylogenetic diversity, composition, structure and dynamics of microbial communities from microcosms under the conditions of different electron donor amendments. The community structures in each of the five alternate electron donor enrichments were significantly shifted in comparison with those of the control microcosm. Commonly existing OTUs between the treatment and control consortia increased from 5 to 17 and more than 50% of OTUs increased around 13.7 to 186 times at least in one of the microcosms after 90-days enrichment. Although the microbial communities at different taxonomic levels were significantly changed by different environmental variable groups in redundancy analysis, significant correlations were observed between the microbial communities and PBDE congener profiles. The lesser-brominated PBDE congeners, tri-BDE congener (BDE-32) and hexa-BDE, were identified as the key factors shaping the microbial community structures at OTU level. Some rare populations, including the known dechlorinating bacterium, Dehalobacter, showed significant positive-correlation with the amounts of PBDE congeners in the consortia. The same results were also observed on some unclassified bacteria. These results suggest that PBDEs-degrading microbial communities can be successfully enriched, and their structures and compositions can be manipulated through adjusting the environmental parameters.

英文摘要Polybrominated diphenyl ethers (PBDEs) can be reductively degraded by microorganisms under anaerobic conditions. However, little is known about the effect of electron donors on microbial communities involved in PBDEs degradation. Here we employed 454 Titanium pyrosequencing to examine the phylogenetic diversity, composition, structure and dynamics of microbial communities from microcosms under the conditions of different electron donor amendments. The community structures in each of the five alternate electron donor enrichments were significantly shifted in comparison with those of the control microcosm. Commonly existing OTUs between the treatment and control consortia increased from 5 to 17 and more than 50% of OTUs increased around 13.7 to 186 times at least in one of the microcosms after 90-days enrichment. Although the microbial communities at different taxonomic levels were significantly changed by different environmental variable groups in redundancy analysis, significant correlations were observed between the microbial communities and PBDE congener profiles. The lesser-brominated PBDE congeners, tri-BDE congener (BDE-32) and hexa-BDE, were identified as the key factors shaping the microbial community structures at OTU level. Some rare populations, including the known dechlorinating bacterium, Dehalobacter, showed significant positive-correlation with the amounts of PBDE congeners in the consortia. The same results were also observed on some unclassified bacteria. These results suggest that PBDEs-degrading microbial communities can be successfully enriched, and their structures and compositions can be manipulated through adjusting the environmental parameters.
WOS标题词Science & Technology
学科主题功能基因组
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME RETARDANTS ; CONTAMINATED AQUIFER ; SP NOV. ; REDUCTIVE DEBROMINATION ; ANAEROBIC DEGRADATION ; GEN. NOV. ; SEDIMENTS ; DIVERSITY ; URANIUM
收录类别SCI
语种英语
WOS记录号WOS:000301640600031
公开日期2012-11-23
源URL[http://ir.qibebt.ac.cn:8080/handle/337004/1424]  
专题青岛生物能源与过程研究所_单细胞中心
作者单位1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Bioenergy Genome Ctr, Qingdao, Shandong, Peoples R China
4.Minist Guangdong Prov Jointly Breeding Base, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China
5.Guangdong Open Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China
6.Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Xu, Meiying,Chen, Xingjuan,Qiu, Mengde,et al. Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments[J]. PLOS ONE,2012,7(1):e30439.
APA Xu, Meiying.,Chen, Xingjuan.,Qiu, Mengde.,Zeng, Xiaowei.,Xu, Jian.,...&Guo, Jun.(2012).Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments.PLOS ONE,7(1),e30439.
MLA Xu, Meiying,et al."Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments".PLOS ONE 7.1(2012):e30439.

入库方式: OAI收割

来源:青岛生物能源与过程研究所

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