A Calcium(II)-based L-arginine for ATP binding and hydrolysis
文献类型：期刊论文
| 作者 | Lv GX(吕功煊) ; Lv GX(吕功煊)
|
| 刊名 | Journal of Inorganic and Organometallic Polymers
 |
| 出版日期 | 2008 |
| 卷号 | 18页码:435-440 |
| 关键词 | ATP Ca(II) Na-4-tosyl-L-arginine methyl ester hydrochloride Recognition Catalytic hydrolysis |
| ISSN号 | 1574-1443 |
| 通讯作者 | 吕功煊 |
| 中文摘要 | The supramolecular recognition of Ca(II) and Na-4-tosyl-L-arginine methyl ester hydrochloride (TAME) with ATP were investigated using 1H and 31P NMR spectra. In the Ca(II)–ATP–TAME ternary system, Ca2+ and TAME bind with ATP via the phosphate chain and adenine ring of ATP. The binding forces are mainly electrostatic and cation (Ca2+)–p and p–p stacking interaction. Furthermore, the hydrolysis of ATP catalyzed by Ca(II) and TAME was studied at pH 7.0 and 60 C using 31P NMR spectra. Kinetics studies show that the ATP hydrolysis rate constant is 0.1035 h-1 in the Ca(II)–TAME–ATP ternary system, whereas the value is 8.5 9 10-3 h-1 under the same conditions without TAME and Ca2+. The Ca(II) ions and TAME accelerate the ATP hydrolysis process about 12-fold. The proposed mechanism of ATP hydrolysis catalyzed by Ca2+–TAME occurs through an addition– elimination reaction sequence. These results can help us get more useful information at the molecular level about the key amino acid residue(s) and metal ions that serve as cofactors in the ATPase effect on ATP hydrolysis/synthesis. |
| 学科主题 | 环境催化与清洁能源 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China (No. 90210027) |
| 语种 | 英语 |
| WOS记录号 | WOS:000260838000001 |
| 公开日期 | 2012-11-09 |
| 源URL | [http://210.77.64.217/handle/362003/1905]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 兰州化学物理研究所_ERC国家工程研究中心 |
| 通讯作者 | Lv GX(吕功煊); Lv GX(吕功煊) |
| 推荐引用方式 GB/T 7714 | Lv GX,Lv GX. A Calcium(II)-based L-arginine for ATP binding and hydrolysis[J]. Journal of Inorganic and Organometallic Polymers,2008,18:435-440. |
| APA | 吕功煊,&吕功煊.(2008).A Calcium(II)-based L-arginine for ATP binding and hydrolysis.Journal of Inorganic and Organometallic Polymers,18,435-440. |
| MLA | 吕功煊,et al."A Calcium(II)-based L-arginine for ATP binding and hydrolysis".Journal of Inorganic and Organometallic Polymers 18(2008):435-440. |
入库方式： OAI收割
来源：兰州化学物理研究所
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