Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotroph from methane and methanol
文献类型：期刊论文
| 作者 | Song H(宋昊) ; Xia CG(夏春谷); Xia CG(夏春谷)
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| 刊名 | J. Natur. Gas Chem.
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| 出版日期 | 2008 |
| 卷号 | 17期号:1页码:103-109 |
| ISSN号 | 1003-9953 |
| 关键词 | poly-3-hydroxybutyrate methanotrophs methane methanol cultivation biosynthesis high molecular weight |
| 通讯作者 | 夏春谷 |
| 中文摘要 | Poly-3-hydroxybutyrate (PHB) can be produced by various species of bacteria. Among the possible carbon sources, both methane and methanol could be a suitable substrate for the production of PHB. Methane is cheap and plentiful not only as natural gas, but also as biogas. Methanol can also maintain methanotrophic activity in some conditions. The methanotrophic strain Methylosinus trichosporium IMV3011 can accumulate PHB with methane and methanol in a brief nonsterile process. Liquid methanol (0.1%) was added to improve the oxidization of methane. The studies were carried out using shake flasks. Cultivation was performed in two stages: a continuous growth phase and a PHB accumulation phase under the conditions short of essential nutrients (ammonium, nitrate, phosphorus, copper, iron (III), magnesium or ethylenediamine tetraacetate (EDTA)) in batch culture. It was found that the most suitable growth time for the cell is 144 h. Then an optimized culture condition for second stage was determined, in which the PHB concentration could be much increased to 0.6 g/L. In order to increase PHB content, citric acid was added as an inhibitor of tricarboxylic acid cycle (TCA). It was found that citric acid is favorable for the PHB accumulation, and the PHB yield was increased to 40% (w/w) from the initial yield of 12% (w/w) after nutrient deficiency cultivation. The PHB produced is of very high quality with molecular weight up to 1.5×106Da. |
| 学科主题 | 清洁催化与生物转化 |
| 资助信息 | the New Century Excellent Talents in University of China (NCET-05−0358);the National Natural Science Foundation of China (20625308);the Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255491700017 |
| 公开日期 | 2012-11-09 |
| 源URL | [http://210.77.64.217/handle/362003/1943]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 通讯作者 | Xia CG(夏春谷); Xia CG(夏春谷) |
| 推荐引用方式 GB/T 7714 | Song H,Xia CG,Xia CG. Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotroph from methane and methanol[J]. J. Natur. Gas Chem.,2008,17(1):103-109. |
| APA | 宋昊,夏春谷,&夏春谷.(2008).Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotroph from methane and methanol.J. Natur. Gas Chem.,17(1),103-109. |
| MLA | 宋昊,et al."Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotroph from methane and methanol".J. Natur. Gas Chem. 17.1(2008):103-109. |
入库方式： OAI收割
来源：兰州化学物理研究所
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