Rapid analysis of multiple components in Radix et Rhizoma Rhei using ultra-performance liquid chromatography tandem mass spectrometry
文献类型：期刊论文
| 作者 | Shi YP(师彦平) ; Shi YP(师彦平)
|
| 刊名 | Acta Chromatography
 |
| 出版日期 | 2011 |
| 卷号 | 23期号:4页码:653-672 |
| 关键词 | ultraperformance liquid chromatography tandem mass spectrometry Radix et Rhizoma Rhei component identification discriminant analysis quality control |
| ISSN号 | 1233-2356 |
| 通讯作者 | 师彦平 |
| 中文摘要 | A simple and rapid method, using online ultraperformance liquid chromatography with photodiode array detection and electrospray ionization mass spectrometry (UPLC–PDA-eλ-ESI–MS/MS), was developed for the in-depth analysis of 50 batches Radix et Rhizoma Rhei. The analysis was performed on a UPLC BEH C18 column using a gradient elution system. Baseline separation could be achieved in less than 7.5 min. At the same time, on the basis of the 50 batches of samples collected from representative cultivated regions, a novel chromatographic fingerprint was devised by UPLC–PDA, in which 27 common peaks were detected and identified by the developed UPLC–MS/MS method step by step according to fragmentation mechanisms, MS/MS data, standards, and relevant literature. Many active components gave prominent [M − H]− ions in the ESI mass spectra. These components include anthraquinones, sennosides, stilbenes, glucose gallates, naphthalenes, and catechins. Furthermore, based on the information of these Radix et Rhizoma Rhei components, and further combined with discriminant analysis, a novel discriminant analysis equation (DAE) was established for the quality control of Radix et Rhizoma Rhei for the first time. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Key Technology Research and Development Program of China (No. 2007BAI37B05);the Western hightech Project Action Plan of the Chinese Academy of Sciences (No. KG-CX2-YW-510) |
| 语种 | 英语 |
| 公开日期 | 2012-10-18 |
| 源URL | [http://210.77.64.217/handle/362003/1523]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Shi YP(师彦平) |
| 推荐引用方式 GB/T 7714 | Shi YP,Shi YP. Rapid analysis of multiple components in Radix et Rhizoma Rhei using ultra-performance liquid chromatography tandem mass spectrometry[J]. Acta Chromatography,2011,23(4):653-672. |
| APA | 师彦平,&师彦平.(2011).Rapid analysis of multiple components in Radix et Rhizoma Rhei using ultra-performance liquid chromatography tandem mass spectrometry.Acta Chromatography,23(4),653-672. |
| MLA | 师彦平,et al."Rapid analysis of multiple components in Radix et Rhizoma Rhei using ultra-performance liquid chromatography tandem mass spectrometry".Acta Chromatography 23.4(2011):653-672. |
入库方式： OAI收割
来源：兰州化学物理研究所
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