Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences
文献类型：期刊论文
| 作者 | Yang SR(杨生荣) ; Wang JQ(王金清); Wang JQ(王金清)
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| 刊名 | Science in China Series B: Chemistry
 |
| 出版日期 | 2009 |
| 卷号 | 52期号:7页码:1009-1013 |
| 关键词 | azobenzene monomer peptide nucleic acid hybridization gold electrode electrochemical biosensors |
| ISSN号 | 1006-9291 |
| 通讯作者 | 王金清 |
| 中文摘要 | Hybridization of peptide nucleic acids probe containing azobenzene (NH2-TNT4, N-PNAs) with DNA was performed by covalently immobilizing of NH2-TNT4 in sequence on the 3-mercaptopropionic acid self-assembled monolayer modified gold electrode with the helps of N-(3-dimethylaminopropy1)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and the hybrid was coded as N-PNAs/DNA. Using [Fe(CN)6] 4−/3− (1:1) as the electrochemical indicator, the electrochemical properties of the N-PNAs self-assembled monolayer (N-PNAs-SAMs) and N-PNAs/DNA hybridization system under the conditions of before and after UV light irradiation were characterized with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectra (EIS). Results showed that the redox currents decreased with the increase of irradiation time, suggesting that the ability of the charge transfer on the electrode surface was weakened and the conformation of hybrid system had been changed, and the control of PNAs/DNA hybridization could be realized by UV light irradiation. |
| 学科主题 | 材料科学与物理化学 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China (Grant No. 50572107);“Top Hundred Talents Program” of Chinese Academy of Sciences |
| 语种 | 英语 |
| 公开日期 | 2012-11-13 |
| 源URL | [http://210.77.64.217/handle/362003/2116]  |
| 专题 | 兰州化学物理研究所_固体润滑国家重点实验室 |
| 通讯作者 | Wang JQ(王金清); Wang JQ(王金清) |
| 推荐引用方式 GB/T 7714 | Yang SR,Wang JQ,Wang JQ. Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences[J]. Science in China Series B: Chemistry,2009,52(7):1009-1013. |
| APA | 杨生荣,王金清,&王金清.(2009).Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences.Science in China Series B: Chemistry,52(7),1009-1013. |
| MLA | 杨生荣,et al."Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences".Science in China Series B: Chemistry 52.7(2009):1009-1013. |
入库方式： OAI收割
来源：兰州化学物理研究所
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