How well do ITS rDNA sequences differentiate species of true morels (Morchella)?
文献类型：期刊论文
| 作者 | Du, Xi-Hui10,11; Zhao, Qi10,11 ; Yang, Zhu L.10,11; Hansen, Karen9; Taskin, Hatira8; Buyukalaca, Saadet8; Dewsbury, Damon6,7; Moncalvo, Jean-Marc6,7; Douhan, Greg W.5; Robert, Vincent A. R. G.4
|
| 刊名 | MYCOLOGIA
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 104期号:6页码:1351-1368 |
| 关键词 | Ascomycota biodiversity biogeography emerencia GCPSR GenBank phylogeny species limits |
| 英文摘要 | Arguably more mycophiles hunt true morels (Morchella) during their brief fruiting season each spring in the northern hemisphere than any other wild edible fungus. Concerns about overharvesting by individual collectors and commercial enterprises make it essential that science-based management practices and conservation policies are developed to ensure the sustainability of commercial harvests and to protect and preserve morel species diversity. Therefore, the primary objectives of the present study were to: (i) investigate the utility of the ITS rDNA locus for identifying Morchella species, using phylogenetic species previously inferred from multilocus DNA sequence data as a reference; and (ii) clarify insufficiently identified sequences and determine whether the named sequences in GenBank were identified correctly. To this end, we generated 553 Morchella ITS rDNA sequences and downloaded 312 additional ones generated by other researchers from GenBank using emerencia and analyzed them phylogenetically. Three major findings emerged: (i) ITS rDNA sequences were useful in identifying 48/62 (77.4%) of the known phylospecies; however, they failed to identify 12 of the 22 species within the species-rich Elata Subclade and two closely related species in the Esculenta Clacle; (ii) at least 66% of the named Morchella sequences in GenBank are misidentified; and (iii) ITS rDNA sequences of up to six putatively novel Morchella species were represented in GenBank. Recognizing the need for a dedicated Web-accessible reference database to facilitate the rapid identification of known and novel species, we constructed Morchella MLST (http://www.cbs.knaw.nl/morchella/), which can be queried with ITS rDNA sequences and those of the four other genes used in our prior multilocus molecular systematic studies of this charismatic genus. |
| 类目[WOS] | Mycology |
| 研究领域[WOS] | Mycology |
| 关键词[WOS] | DNA-SEQUENCES ; PHYLOGENETIC-RELATIONSHIPS ; INTRAGENOMIC VARIATION ; KINGDOM FUNGI ; RIBOSOMAL DNA ; DIVERSITY ; FUSARIUM ; IDENTIFICATION ; DATABASES ; RECOGNITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312361100009 |
| 公开日期 | 2012-12-04 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/15798]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA 2.USDA ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA 3.USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA 4.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands 5.Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA 6.Royal Ontario Museum, Dept Nat Hist, Toronto, ON M5S 2C6, Canada 7.Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 2C6, Canada 8.Cukurova Univ, Fac Agr, Dept Hort, TR-01330 Adana, Turkey 9.Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden 10.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Xi-Hui,Zhao, Qi,Yang, Zhu L.,et al. How well do ITS rDNA sequences differentiate species of true morels (Morchella)?[J]. MYCOLOGIA,2012,104(6):1351-1368. |
| APA | Du, Xi-Hui.,Zhao, Qi.,Yang, Zhu L..,Hansen, Karen.,Taskin, Hatira.,...&O'Donnell, Kerry.(2012).How well do ITS rDNA sequences differentiate species of true morels (Morchella)?.MYCOLOGIA,104(6),1351-1368. |
| MLA | Du, Xi-Hui,et al."How well do ITS rDNA sequences differentiate species of true morels (Morchella)?".MYCOLOGIA 104.6(2012):1351-1368. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。