Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011
文献类型：期刊论文
| 作者 | Xia CG(夏春谷)
|
| 刊名 | World J Microbiol Biotechnol
 |
| 出版日期 | 2010 |
| 卷号 | 26页码:701-708 |
| 关键词 | Epoxypropane Methanotrophs Methanol Methane monooxygenase |
| ISSN号 | 1528-7483 |
| 通讯作者 | 辛嘉英 |
| 中文摘要 | Methanotrophs containing methane monooxygenase (MMO) can catalyze the epoxidation of propene to epoxypropane. Methane cannot support dense biomass growth due to its low aqueous solubility. Low growth rate is important limiting factor for the application of methanotrophs. Methanol can act as growth substrate, but direct addition of methanol is toxic to most methanotrophs. The MMO activity during growth on methanol is also uncertain. In this paper, methanol-adapted Methylosinus trichosporium IMV 3011 was successfully cultivated at high cell densities using methanol as sole carbon source. A biomass density of 1.68 g dry weight cell l-1 was achieved and cells contained almost 80% of the MMO activity measured for cells grown with methane. It has been found that methanol can also act as the electron-donating substrate to regenerate the NADH and drive epoxypropane synthesis. The effect of methanol supply on the epoxidation capacity of Methylosinus trichosporium IMV3011 was studied in batch reactor. 0.016% methanol concentration was found to give the highest propene epoxidation capacity. |
| 学科主题 | 物理化学 |
| 资助信息 | the National Natural Science Foundation of China (20873034);the Program for New Century Excellent Talents in University (NCET-05-0356);the Open Project Program of the State Key Laboratory of Bioreactor Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000275464900015 |
| 公开日期 | 2012-11-30 |
| 源URL | [http://210.77.64.217/handle/362003/2272]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xia CG. Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011[J]. World J Microbiol Biotechnol,2010,26:701-708. |
| APA | 夏春谷.(2010).Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011.World J Microbiol Biotechnol,26,701-708. |
| MLA | 夏春谷."Epoxypropane biosynthesis by whole cell suspension of methanol-growth Methylosinus trichosporium IMV 3011".World J Microbiol Biotechnol 26(2010):701-708. |
入库方式： OAI收割
来源：兰州化学物理研究所
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