Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography
文献类型：期刊论文
| 作者 | Lv Qiaoying; Yang Yi; Zhao Yongxin; Gu Dongyu; He Dajun; Yili Abulimiti ; Ma Qingling; Cheng Zhen; Gao Yanhua; Aisa Haji Akber
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| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2009 |
| 卷号 | 32期号:19页码:2879-2892 |
| 关键词 | Biosynthesis Chickpea High speed countercurrent chromatography Isoflavone Seed Sprout |
| ISSN号 | 10826076 |
| 英文摘要 | Chickpea is known as a plant that is rich in protein, carbohydrates, and nutrition, and its seeds and sprouts have been processed into various health foods. In the present study, four isoflavones were purified from the seeds and sprouts of chickpea by high-speed countercurrent chromatography (HSCCC) using two biphasic solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:5:5, v/v) and ethyl acetate-water (1:1, v/v). The results indicated that 14.2mg of formononetin, 15.7mg of biochanin A, 9.1mg of ononin, 11.3mg of biochanin A-7-O-β- D-glucoside were obtained from 150mg of sprout extracts with the purity of 92.26%, 95.86%, 95.32%, and 96.56%, respectively. Compared with the sprouts, separation of seed extracts yielded less amounts of biochanin A-7-O-β - D-glucoside and biochanin A with lower purity. The results indicate that four main isoflavones in chickpea, i.e., isoflavones, formononetin, biochanin A, ononin, and biochanin A-7-O-β- D-glucoside, are substantially increased by biosynthesis during the seed germination. © Taylor & Francis Group, LLC. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000270812700009 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1778]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
| 作者单位 | NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv Qiaoying,Yang Yi,Zhao Yongxin,et al. Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(19):2879-2892. |
| APA | Lv Qiaoying.,Yang Yi.,Zhao Yongxin.,Gu Dongyu.,He Dajun.,...&Ito Yoichiro.(2009).Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(19),2879-2892. |
| MLA | Lv Qiaoying,et al."Comparative Study on Separation and Purification of Isoflavones from the Seeds and Sprouts of Chickpea by High-Speed Countercurrent Chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.19(2009):2879-2892. |
入库方式： OAI收割
来源：新疆理化技术研究所
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