Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques
文献类型：期刊论文
| 作者 | Yang Chunyan; Yang Yi; Aisa Haji Akber; Xin Xuelei ; Ma Hairong; Yili Abulimiti; Zhao Yongxin
|
| 刊名 | Journal of Separation Science
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:8页码:977-983 |
| 关键词 | Antioxidants Astragalus altaicus Bunge Bioassay-guided isolation Combination of chromatographic techniques Flavonoids High-speed counter-current chromatography |
| ISSN号 | 16159306 |
| 英文摘要 | An efficient method for bioassay-guided preparative isolation of antioxidants from the n-butanol extract of Astragalus altaicus Bunge was ingeniously developed by combination of silica gel column chromatography and high-speed counter-current chromatography. Under the bioassay-guidance of antioxidant activities, the antioxidants were gradually separated from the crude sample of Astragalus altaicus Bunge by silica gel column chromatography and high-speed counter-current chromatography. Silica gel column chromatography separation was performed with chloroform, chloroform-methanol (100:1∼5:1, v/v) and chloroform-methanol-water (5:1:0.1∼2:1:0.1, v/v/v). High-speed counter-current chromatography separation was performed with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:1:6, v/v/v), which was successfully selected by thin layer chromatography analysis, at a flow rate of 1.5 mL/min. As a result, isorhamnetin-3-gentiobioside (20.8 mg), rutin (82.0 mg), and narcissin (12.8 mg) were obtained for the first time from 200 mg of the crude sample, ABS-5 of Astragalus altaicus Bunge. The purities were all at over 95% by high-performance liquid chromatography analysis, and their structures were unambiguously identified by mass spectroscopy, 1H, and 13C nuclear magnetic resonance spectroscopy. Antioxidant activities of the three compounds were also assayed by in vitro ABTS [2,2'-azino-bis-(3- ethylbenzothiazoline-6-sulphonic acid) diamonium salt] radical cation scavenging activity. Among them, rutin possessed the highest antioxidant capacity with SC50 value of 22.15 μg/mL. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| 学科主题 | Chemistry |
| WOS记录号 | WOS:000304038200009 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1398]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China;China Three Gorges Univ, Coll Med, Yichang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Chunyan,Yang Yi,Aisa Haji Akber,et al. Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques[J]. Journal of Separation Science,2012,35(8):977-983. |
| APA | Yang Chunyan.,Yang Yi.,Aisa Haji Akber.,Xin Xuelei.,Ma Hairong.,...&Zhao Yongxin.(2012).Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques.Journal of Separation Science,35(8),977-983. |
| MLA | Yang Chunyan,et al."Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques".Journal of Separation Science 35.8(2012):977-983. |
入库方式： OAI收割
来源：新疆理化技术研究所
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