studies on the effect of column angle in figure-8 centrifugal counter-current chromatography
文献类型：期刊论文
| 作者 | Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro |
| 刊名 | Journal of Chromatography A
 |
| 出版日期 | 2011 |
| 卷号 | 1218期号:36页码:6128-6134 |
| 关键词 | Centrifugal counter-current chromatography Figure-8 column Angle between column axis and centrifugal force Retention of the stationary phase Resolution Dipeptide DNP-amino acid |
| ISSN号 | 219673 |
| 英文摘要 | The performance of the figure-8 column configuration in centrifugal counter-current chromatography was investigated by changing the angle between the column axis (a line through the central post and the peripheral post on which the figure-8 coil is wound) and the centrifugal force. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was changed from 0° (column axis parallel to the centrifugal force) to 45° and 45° to 90° (column axis perpendicular to the centrifugal force), peak resolution (Rs) changed from 1.93 (Sf = 37.8%) to 1.54 (Sf = 30.6%), then to 1.31 (Sf = 40.5%) with the lower mobile phase and from 1.21 (Sf = 38.8%) to 1.10 (Sf = 34.4%), then to 0.99 (Sf = 42.2%) with the upper mobile phase, respectively, where the stationary phase retention, Sf, is given in parentheses. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1. M hydrochloric acid (1:1:1:1, v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 0.05. ml/min at 1000. rpm. When the column angle was altered from 0° to 45° and 45° to 90°, Rs changed from 1.77 (1st peak/2nd peak) and 1.52 (2nd peak/3rd peak) (Sf = 27.3%) to 1.24 and 1.02 (Sf = 35.4%), then to 1.69 and 1.49 (Sf = 42.1%) with the lower mobile phase, and from 1.73 and 0.84 (SF = 41.2%) to 1.44 and 0.73 (Sf = 45.6%), then to 1.21 and 0.63 (Sf = 55.6%) with the upper mobile phase, respectively. The performance of figure-8 column at 0° and 90° was also compared at different flow rates. The results show that Rs was increased with decreased flow rate yielding the highest value at the 0° column angle with lower mobile phase. The overall results of our studies indicated that a 0° column angle for the figure-8 column enhances the mixing of two phases in the column to improve peak resolution while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000294523200018 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1458]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
| 作者单位 | NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. studies on the effect of column angle in figure-8 centrifugal counter-current chromatography[J]. Journal of Chromatography A,2011,1218(36):6128-6134. |
| APA | Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).studies on the effect of column angle in figure-8 centrifugal counter-current chromatography.Journal of Chromatography A,1218(36),6128-6134. |
| MLA | Yang Yi,et al."studies on the effect of column angle in figure-8 centrifugal counter-current chromatography".Journal of Chromatography A 1218.36(2011):6128-6134. |
入库方式： OAI收割
来源：新疆理化技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。