Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection
文献类型：期刊论文
| 作者 | Pang Nan-Nan; Zhou Yu; Bai Yu; Liao Jie; Liu Hu-Wei |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 39期号:2页码:231-234 |
| 关键词 | Liquid chromatography Mass spectrometry Microelectro purification device Ultra-filtration Recombinant human erythropoietin |
| ISSN号 | 0253-3820 |
| 英文摘要 | Microelectro purification device (MEPD) constructed by our laboratory could obtain online desalting of recombinant human erythropoietin (rhEPO) in 2 min with high efficiency. Electrospray mass spectrometry with positive ion mode was used for the detection. Compared with traditional methods, such as gradient elution by C(18)-reversed phase liquid chromatography (150 mm X4. 6 mm, 5 mu m) as well as ultra-filtration, MEPD could further enhance the signal to noise ratio of rhEPO and the detection sensitivity. It could also shorten the analysis time dramatically. This simple and rapid method could be a promising pretreatment candidate for the rhEPO analysis of mass spectrometry. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| WOS记录号 | WOS:000290240800015 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1672]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
| 作者单位 | Peking Univ, Coll Chem & Chem Engn, Beijing Natl Lab Mol Sci, Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China;Chinese Acad Sci, Key Lab Plant Resources & Chem Arid Zone, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Gen Hosp Chinese Peoples Liberat Army, Med Expt & Anal Ctr, Beijing 100853, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pang Nan-Nan,Zhou Yu,Bai Yu,et al. Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(2):231-234. |
| APA | Pang Nan-Nan,Zhou Yu,Bai Yu,Liao Jie,&Liu Hu-Wei.(2011).Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(2),231-234. |
| MLA | Pang Nan-Nan,et al."Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.2(2011):231-234. |
入库方式： OAI收割
来源：新疆理化技术研究所
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